PEPITE EA4267, Labex LipSTIC, Univ. Bourgogne Franche-Comté, F-25000 Besançon, France.
Molecular Mechanisms in Neurodegenerative Dementia Laboratory (MMDN), INSERM, U1198, Environmental Impact in Alzheimer's Disease and Related Disorders (EiAlz) Team, 34095 Montpellier, France; University of Montpellier, 34095 Montpellier, France; EPHE, 75014 Paris, France.
Colloids Surf B Biointerfaces. 2021 Jan;197:111432. doi: 10.1016/j.colsurfb.2020.111432. Epub 2020 Oct 27.
Oil-in-water nanoemulsions are used in numerous biomedical applications as delivery systems. The droplet size in the nanometer range and their composition were extensively developed for carrying and enhancing the absorption of lipophilic drugs and lipids of interest. In the present study, critical parameters involved in the spontaneous nanoemulsification process such as the temperature, the oil type, the surfactant-to-oil and water-to-oil ratios were investigated. The aim was to design a solvent-free procedure for the spontaneous nanoemulsification at a low temperature of a large variety of triglycerides including vegetable oils. Nanoemulsification of medium-chain triglyceride (MCT) was not dependent on the temperature while nanodroplets of long-chain triglycerides (LCT) were only obtained by reaching the cloud point of ethoxylated surfactant Kolliphor® HS15. The molar volume of triglycerides was considered as a predictive parameter governing both, the spontaneous nanoemulsification at low temperature and the Ostwald ripening rate. The physical mixture of MCT and LCT was a promising strategy to prepare stable and fine nanoemulsions at 37 °C. They were characterized by a hydrodynamic diameter comprised between 20 and 30 nm and a narrow size distribution. These findings pave the way to new applications for the parenteral nutrition and the delivery of thermosensitive drugs and lipophilic molecules such as antioxidants.
水包油型纳米乳在许多生物医药应用中被用作输送系统。纳米范围内的液滴尺寸及其组成被广泛开发用于携带和增强亲脂性药物和感兴趣的脂类的吸收。在本研究中，研究了自发纳米乳化过程中涉及的关键参数，如温度、油的类型、表面活性剂与油的比例以及水与油的比例。目的是设计一种无溶剂的程序，在低温下对包括植物油在内的各种甘油三酯进行自发纳米乳化。中链甘油三酯 (MCT) 的纳米乳化不依赖于温度，而只有当乙氧基化表面活性剂 Kolliphor® HS15 达到浊点时，长链甘油三酯 (LCT) 的纳米液滴才会形成。甘油三酯的摩尔体积被认为是控制低温下自发纳米乳化和奥斯特瓦尔德熟化速率的预测参数。MCT 和 LCT 的物理混合物是在 37°C 下制备稳定和精细纳米乳的一种很有前途的策略。它们的特征是水动力学直径在 20 至 30nm 之间，且分布较窄。这些发现为肠外营养以及热敏药物和脂溶性分子（如抗氧化剂）的输送开辟了新的应用途径。
