Yukird Jutiporn, Chailapakul Orawon, Rodthongkum Nadnudda
Nanoscience and Technology Program, Graduate School, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok, 10330, Thailand.
Electrochemistry and Optical Spectroscopy Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok, 10330, Thailand.
Talanta. 2021 Jan 15;222:121561. doi: 10.1016/j.talanta.2020.121561. Epub 2020 Aug 25.
A label-free electrochemical immunosensor based on polyaniline (PANI) micellar electrode was firstly fabricated for direct AMH detection. To control the size regularity of PANI, a micelle-based method using ammonium peroxydisulfate (APS) as a reducing agent was employed in the polymerization process. The Anti-AMH antibodies were readily immobilized onto PANI via peptide bond to enhance the sensor specificity and sensitivity. This sensor was applied for the detection of AMH, an ovarian response indicator in female related to residual eggs during a woman's monthly cycle. The sensor performances were systematically investigated by differential pulse voltammetry. The anodic peak current decreases with the increase of AMH concentration owing to blocking of electron transfer by AMH. Under the optimal conditions, this sensor offers high sensitivity with a low detection limit of 0.1 ng mL and a wide linear range of 0.1-4 ng mL, which is sensitive enough to indicate the ability to produce eggs during a woman's monthly cycle. Furthermore, this system requires lower sample volume (5 μL), while offers the simple fabrication with low cost and no synthetic challenge and faster analysis compared with a standard ELISA. Ultimately, this sensor was successfully applied for the detection of AMH in human serum with satisfactory results. Thus, it might be an alternative tool for AMH screening in clinical setting.
首次制备了基于聚苯胺（PANI）胶束电极的无标记电化学免疫传感器用于直接检测抗缪勒管激素（AMH）。为了控制聚苯胺的尺寸规整性，在聚合过程中采用了以过硫酸铵（APS）作为还原剂的基于胶束的方法。抗AMH抗体通过肽键轻松固定在聚苯胺上，以提高传感器的特异性和灵敏度。该传感器用于检测AMH，AMH是女性卵巢反应指标，与女性月经周期中的剩余卵子有关。通过差分脉冲伏安法系统研究了传感器性能。由于AMH对电子转移的阻断，阳极峰值电流随AMH浓度的增加而降低。在最佳条件下，该传感器具有高灵敏度，检测限低至0.1 ng/mL，线性范围宽达0.1 - 4 ng/mL，足以灵敏地指示女性月经周期中的产卵能力。此外，该系统所需样品体积较小（5 μL），与标准酶联免疫吸附测定法（ELISA）相比，具有制备简单、成本低、无合成难题且分析速度更快的特点。最终，该传感器成功应用于检测人血清中的AMH，结果令人满意。因此，它可能是临床环境中AMH筛查的替代工具。
