Institute of Plasma Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
University of Science and Technology of China, Hefei 230026, China.
Molecules. 2020 Nov 5;25(21):5141. doi: 10.3390/molecules25215141.
The objective of this study was to investigate the stability and degradation kinetics of -acetylneuraminic acid (Neu5Ac). The pH of the solution strongly influenced the stability of Neu5Ac, which was more stable at neutral pH and low temperatures. Here, we provide detailed information on the degradation kinetics of Neu5Ac at different pH values (1.0, 2.0, 11.0 and 12.0) and temperatures (60, 70, 80 and 90 °C). The study of the degradation of Neu5Ac under strongly acidic conditions (pH 1.0-2.0) is highly pertinent for the hydrolysis of polysialic acid. The degradation kinetics of alkaline deacetylation were also studied. Neu5Ac was highly stable at pH 3.0-10.0, even at high temperature, but the addition of HO greatly reduced its stability at pH 5.0, 7.0 and 9.0. Although Neu5Ac has a number of applications in products of everyday life, there are no reports of rigorous shelf-life studies. This research provides kinetic data that can be used to predict product shelf lives at different temperatures and pH values.
本研究旨在探讨β-乙酰神经氨酸（Neu5Ac）的稳定性和降解动力学。溶液的 pH 值强烈影响 Neu5Ac 的稳定性，其在中性 pH 值和低温下更稳定。在此，我们提供了在不同 pH 值（1.0、2.0、11.0 和 12.0）和温度（60、70、80 和 90°C）下 Neu5Ac 降解动力学的详细信息。在强酸性条件（pH 1.0-2.0）下研究 Neu5Ac 的降解对于多糖唾液酸的水解非常重要。还研究了碱性脱乙酰基的降解动力学。Neu5Ac 在 pH 值 3.0-10.0 下非常稳定，即使在高温下也是如此，但在 pH 值 5.0、7.0 和 9.0 下添加 HO 会大大降低其稳定性。尽管 Neu5Ac 在日常生活用品中有多种应用，但没有关于严格保质期研究的报道。这项研究提供了可以用于预测不同温度和 pH 值下产品保质期的动力学数据。
