Olejarz Patrycja, Chwatko Grażyna, Kubalczyk Paweł, Purgat Krystian, Głowacki Rafał, Borowczyk Kamila
Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, 163 Pomorska Str., 90-236 Łódź, Poland.
Pharmaceuticals (Basel). 2020 Nov 5;13(11):367. doi: 10.3390/ph13110367.
Tenofovir disoproxil fumarate is widely used in the therapy of human immunodeficiency virus and hepatitis B virus; however, a high concentration of the prodrug effects kidney function damage. To control the effectiveness of kidney functions in treated patients, the level of creatinine in the body must be controlled. This work describes a simple, fast, and "plastic-waste" reducing method for the simultaneous determination of tenofovir and creatinine in human urine and plasma. In both assays, only 50 µL of body fluid was required. The tests were carried out by reversed phase high-performance liquid chromatography with UV detection. In urine samples, the limits of detection for tenofovir and creatinine were 4 µg mL and 0.03 µmol mL, respectively. In plasma samples, the limits of detection were 0.15 µg mL for tenofovir and 0.0003 µmol mL for creatinine. The method was applied for the determination of tenofovir and creatinine in human urine and plasma samples. The biggest advantage of the elaborated method is the possibility to determine tenofovir and creatinine in one analytical run in both urine and plasma sample collected from HIV and HBV patients. The possibility to reduce the level of laboratory waste in a sample preparation protocol is in the mainstream of a new trend of analytical chemistry which is based on green chemistry.
富马酸替诺福韦二吡呋酯广泛应用于人类免疫缺陷病毒和乙型肝炎病毒的治疗；然而，高浓度的前药会损害肾功能。为了控制接受治疗患者的肾功能有效性，必须控制体内肌酐水平。本文描述了一种简单、快速且能减少“塑料废物”的方法，用于同时测定人尿液和血浆中的替诺福韦和肌酐。在这两种分析中，仅需50 μL体液。测试通过反相高效液相色谱法结合紫外检测进行。在尿液样本中，替诺福韦和肌酐的检测限分别为4 μg/mL和0.03 μmol/mL。在血浆样本中，替诺福韦的检测限为0.15 μg/mL，肌酐的检测限为0.0003 μmol/mL。该方法应用于测定人类尿液和血浆样本中的替诺福韦和肌酐。所阐述方法的最大优点是能够在一次分析运行中同时测定从艾滋病毒和乙肝患者采集的尿液和血浆样本中的替诺福韦和肌酐。在样品制备方案中降低实验室废物水平的可能性符合基于绿色化学的分析化学新趋势的主流。
