Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, Canada.
Department of Biology, Western University, London, Ontario, Canada.
Biochem Soc Trans. 2020 Dec 18;48(6):2779-2789. doi: 10.1042/BST20200660.
Polycomb repressive complex 2 (PRC2) is an evolutionally conserved multisubunit complex essential for the development of eukaryotes. In Arabidopsis thaliana (Arabidopsis), CURLY LEAF (CLF) and SWINGER (SWN) are PRC2 catalytic subunits that repress gene expression through trimethylating histone H3 at lysine 27 (H3K27me3). CLF and SWN function to safeguard the appropriate expression of key developmental regulators throughout the plant life cycle. Recent researches have advanced our knowledge of the biological roles and the regulation of the activity of CLF and SWN. In this review, we summarize these recent findings and highlight the redundant and differential roles of CLF and SWN in plant development. Further, we discuss the molecular mechanisms underlying CLF and SWN recruitment to specific genomic loci, as well as their interplays with Trithorax-group (TrxG) proteins in plants.
多梳抑制复合物 2（PRC2）是一个进化上保守的多亚基复合物，对于真核生物的发育至关重要。在拟南芥（Arabidopsis）中，卷曲叶（CLF）和SWINGER（SWN）是 PRC2 的催化亚基，通过将组蛋白 H3 赖氨酸 27 三甲基化（H3K27me3）来抑制基因表达。CLF 和 SWN 的功能是确保关键发育调节剂在植物整个生命周期中的适当表达。最近的研究提高了我们对 CLF 和 SWN 的生物学作用和活性调节的认识。在这篇综述中，我们总结了这些最新发现，并强调了 CLF 和 SWN 在植物发育中的冗余和差异作用。此外，我们讨论了 CLF 和 SWN 招募到特定基因组位点的分子机制，以及它们与植物中 Trithorax-group（TrxG）蛋白的相互作用。
