Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, Haryana, India.
Department of Molecular Biology, Biotechnology and Bioinformatics CCS Haryana Agricultural University, Hisar, Haryana, India.
Comb Chem High Throughput Screen. 2021;24(10):1769-1783. doi: 10.2174/1386207323666201110144542.
Novel coronavirus SARS-CoV-2 is responsible for the COVID-19 pandemic. It was first reported in Wuhan, China, in December 2019, and despite the tremendous efforts to control the disease, it has now spread almost all over the world. The interaction of SARSCoV- 2spike protein and its acceptor protein ACE2 is an important issue in determining viral host range and cross-species infection, while the binding capacity of spike protein to ACE2 of different species is unknown.
The present study has been conducted to determine the susceptibility of livestock, poultry and pets to SARS-CoV-2.
We evaluated the receptor-utilizing capability of ACE2s from various species by sequence alignment, phylogenetic clustering and protein-ligand interaction studies with the currently known ACE2s utilized by SARS-CoV-2.
In-silico study predicted that SARS-CoV-2 tends to utilize ACE2s of various animal species with varied possible interactions. The probability of the receptor utilization will be greater in horse and poor in chicken, followed by ruminants.
Present study predicted that SARS-CoV-2 tends to utilize ACE2s of various livestock and poultry species with greater probability in equine and poor in chicken. The study may provide important insights into the animal models for SARS-CoV-2 and animal management for COVID- 19 control.
新型冠状病毒 SARS-CoV-2 引发了 COVID-19 大流行。该病毒于 2019 年 12 月在中国武汉首次报告，尽管已采取巨大努力来控制该疾病，但目前它已几乎传播至世界各地。SARS-CoV-2 刺突蛋白与其受体蛋白 ACE2 的相互作用是决定病毒宿主范围和种间感染的重要问题，而刺突蛋白与不同物种 ACE2 的结合能力尚不清楚。
本研究旨在确定家畜、家禽和宠物对 SARS-CoV-2 的易感性。
我们通过序列比对、系统发育聚类以及与 SARS-CoV-2 目前已知的 ACE2 进行蛋白-配体相互作用研究，评估了来自不同物种的 ACE2 受体利用能力。
计算机模拟研究预测，SARS-CoV-2 倾向于利用各种动物物种的 ACE2，可能存在不同的相互作用。在马中，这种受体利用的可能性更大，而在鸡中则较小，反刍动物介于两者之间。
本研究预测，SARS-CoV-2 倾向于利用各种家畜和家禽物种的 ACE2，在马中利用的可能性更大，而在鸡中则较小。该研究可能为 SARS-CoV-2 的动物模型以及 COVID-19 防控中的动物管理提供重要的见解。
