Queen's University Belfast Wellcome-Wolfson Institute for Experimental Medicine, Belfast, UK
Emergency Department, Children's Health Ireland at Temple Street, Dublin D01 YC67, Ireland.
Arch Dis Child. 2021 Jul;106(7):680-686. doi: 10.1136/archdischild-2020-320558. Epub 2020 Nov 10.
Studies based on molecular testing of oral/nasal swabs underestimate SARS-CoV-2 infection due to issues with test sensitivity, test timing and selection bias. The objective of this study was to report the presence of SARS-CoV-2 antibodies, consistent with previous infection.
This multicentre observational cohort study, conducted between 16 April to 3 July 2020 at 5 UK sites, recruited children of healthcare workers, aged 2-15 years. Participants provided blood samples for SARS-CoV-2 antibody testing and data were gathered regarding unwell contacts and symptoms.
1007 participants were enrolled, and 992 were included in the final analysis. The median age of participants was 10·1 years. There were 68 (6.9%) participants with positive SARS-CoV-2 antibody tests indicative of previous SARS-CoV-2 infection. Of these, 34/68 (50%) reported no symptoms prior to testing. The presence of antibodies and the mean antibody titre was not influenced by age. Following multivariable analysis four independent variables were identified as significantly associated with SARS-CoV-2 seropositivity: known infected household contact OR=10.9 (95% CI 6.1 to 19.6); fatigue OR=16.8 (95% CI 5.5 to 51.9); gastrointestinal symptoms OR=6.6 (95% CI 3.0 to 13.8); and changes in sense of smell or taste OR=10.0 (95% CI 2.4 to 11.4).
Children demonstrated similar antibody titres in response to SARS-CoV-2 irrespective of age. Fatigue, gastrointestinal symptoms and changes in sense of smell or taste were the symptoms most strongly associated with SARS-CoV-2 antibody positivity.
NCT0434740.
基于口腔/鼻腔拭子的分子检测的研究由于检测灵敏度、检测时机和选择偏倚等问题，低估了 SARS-CoV-2 感染。本研究的目的是报告与既往感染一致的 SARS-CoV-2 抗体的存在情况。
这项多中心观察性队列研究于 2020 年 4 月 16 日至 7 月 3 日在英国 5 个地点进行，招募了医护人员的 2-15 岁儿童。参与者提供了 SARS-CoV-2 抗体检测的血液样本，并收集了有关不适接触者和症状的数据。
共纳入 1007 名参与者，最终有 992 名参与者纳入最终分析。参与者的中位年龄为 10.1 岁。有 68 名（6.9%）参与者 SARS-CoV-2 抗体检测呈阳性，表明有既往 SARS-CoV-2 感染。其中，34/68（50%）人在检测前无症状。抗体的存在和平均抗体滴度不受年龄影响。多变量分析后，有四个独立变量被确定为与 SARS-CoV-2 血清阳性显著相关：已知感染的家庭接触者 OR=10.9（95%CI 6.1 至 19.6）；疲劳 OR=16.8（95%CI 5.5 至 51.9）；胃肠道症状 OR=6.6（95%CI 3.0 至 13.8）；以及嗅觉或味觉改变 OR=10.0（95%CI 2.4 至 11.4）。
儿童对 SARS-CoV-2 的抗体反应相似，与年龄无关。疲劳、胃肠道症状和嗅觉或味觉改变是与 SARS-CoV-2 抗体阳性最相关的症状。
NCT0434740。
