Marquardt O, Heermann K H, Seifer M, Gerlich W H
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Postgrad Med J. 1987;63 Suppl 2:41-50.
The human hepatoma cell PLC/PRF/5 contains cloned genomes of hepatitis B virus, one of which was transfected to mouse cells (LTK-) or to human carcinoma cells (HeLa). Expression of the viral surface proteins was measured using monoclonal antibodies. The large protein with the pre-S1 domain was best expressed in HeLa cells and least in LTK- cells. In vivo growth of parental hepatoma cells or transfected HeLa cells increased expression of large, and decreased expression of small protein. Secretion of large protein required an excess of small protein. The secreted HBsAg from transfected HeLa cells contained filaments. Since the virus contains a defined proportion of all three proteins, a special regulatory situation is necessary for maturation of its envelope and its secretion in vivo.
人肝癌细胞PLC/PRF/5含有乙型肝炎病毒的克隆基因组，其中一个基因组被转染到小鼠细胞（LTK-）或人癌细胞（HeLa）中。使用单克隆抗体测量病毒表面蛋白的表达。带有前S1结构域的大蛋白在HeLa细胞中表达最好，在LTK-细胞中表达最少。亲代肝癌细胞或转染的HeLa细胞在体内生长会增加大蛋白的表达，并降低小蛋白的表达。大蛋白的分泌需要过量的小蛋白。转染的HeLa细胞分泌的HBsAg含有丝状结构。由于该病毒包含所有三种蛋白的特定比例，因此其包膜成熟及其在体内的分泌需要一种特殊的调节情况。
