Department of Orthopaedics, QingPu Branch of Zhongshan Hospital Affiliated To Fudan University, No. 1158 Park East Road, Qingpu District, Shanghai, 201700, China.
Mol Cell Biochem. 2021 Feb;476(2):831-839. doi: 10.1007/s11010-020-03948-8. Epub 2020 Nov 10.
Rheumatoid arthritis (RA) is a long-standing and growing autoimmune disease. Therefore, the present study was intended to investigate the effect of Corynoline (COR) on CFA induced rheumatoid arthritis in a rat model. Results suggested that COR causes significant reduction in paw swelling, edema, arthritis score, thymus and spleen indexes and neutrophil infiltration (p < 0.01). Moreover, the levels of inflammatory cytokines (interleukin- 1β, -6, and -17, and TNF-α) and anti-collagen II-specific immunoglobulins (IgG1 and IgG2a) were decreased significantly (p < 0.01) together with increase in antioxidant enzymes (SOD, CAT, and GSH) (p < 0.01) in COR-treated group in dose-dependent manner. In western blot analysis, COR-treated group showed concentration-dependent reduction of expression of COX-2, 5-LOX and NF-p65 as compared to CFA rats. Moreover, COR-treated group showed mild inflammation of cartilage with fewer cartilage erosion and synovititis with most significant reversal of arthritic features in the rats treated with 30 mg/kg. It has been concluded that, COR alleviates oxidative stress and inflammation in arthritic rats, thus verifying its anti-rheumatoid arthritis property.
类风湿性关节炎（RA）是一种长期存在且不断发展的自身免疫性疾病。因此，本研究旨在探讨苦参碱（COR）对 CFA 诱导的大鼠类风湿关节炎模型的影响。结果表明，COR 可显著减轻爪肿胀、水肿、关节炎评分、胸腺和脾脏指数以及中性粒细胞浸润（p<0.01）。此外，COR 处理组的炎症细胞因子（白细胞介素-1β、-6、-17 和 TNF-α）和抗胶原 II 特异性免疫球蛋白（IgG1 和 IgG2a）水平显著降低（p<0.01），同时抗氧化酶（SOD、CAT 和 GSH）水平显著升高（p<0.01），呈剂量依赖性。在 Western blot 分析中，与 CFA 大鼠相比，COR 处理组 COX-2、5-LOX 和 NF-κB p65 的表达呈浓度依赖性降低。此外，COR 处理组的软骨炎症较轻，软骨侵蚀和滑膜炎较少，在 30mg/kg 剂量下治疗的大鼠关节炎特征得到了最显著的逆转。综上所述，COR 可减轻关节炎大鼠的氧化应激和炎症，从而验证了其抗类风湿性关节炎的特性。
