Rodgher Suzelei, Contador Thais M, Rocha Giseli S, Espindola Evaldo L G
Universidade Estadual Paulista/UNESP, Instituto de Ciência e Tecnologia (UNESP/SJC), Departamento de Engenharia Ambiental, Parque Tecnológico de São José dos Campos, Estrada Dr. Altino Bondensan, 500, 12247-016 São José dos Campos, SP, Brazil.
Universidade de São Paulo, Escola de Engenharia de São Carlos, Avenida Trabalhador São Carlense, 400, 13566-590 São Carlos, SP, Brazil.
An Acad Bras Cienc. 2020 Nov 6;92(suppl 2):e20190050. doi: 10.1590/0001-3765202020190050. eCollection 2020.
The aim of this study was to evaluate the effect of phosphorus (P) on the toxicity of zinc (Zn) for the alga Raphidocelis subcapitata. P was provided in three concentrations: 2.3 x 10-4 mol L-1, 2.3 x 10-6 mol L-1 and 1.0 x 10-6 mol L-1. Algal cells were acclimated to the specific P concentrations before the start of the experiment. The chemical equilibrium software MINEQL+ 4.61 was employed to calculate the Zn2+ concentration. After acclimated, the algal cells were inoculated into media containing different Zn concentrations (0.09 x 10-6 mol L-1 to 9.08 x 10-6 mol L-1). The study showed that besides the reduction in algal growth rates, phosphorus had an important influence on the toxicity of zinc for microalga. The inhibitory Zn2+ concentration values for R. subcapitata were 2.74 x 10-6 mol L-1, 0.58 x 10-6 mol L-1 and 0.24 x 10-6 mol L-1 for the microalgae acclimated at P concentrations of 2.3 x 10-4 mol L-1, 2.3 x 10-6 mol L-1 and 1.0 x 10-6 mol L-1, respectively. Ecotoxicological studies should consider the interaction between metal concentrations and varying P values to provide realistic data of what occurs in phytoplankton communities in environments.
本研究的目的是评估磷（P）对锌（Zn）对藻类小头莱茵衣藻毒性的影响。提供了三种磷浓度：2.3×10⁻⁴ mol L⁻¹、2.3×10⁻⁶ mol L⁻¹和1.0×10⁻⁶ mol L⁻¹。在实验开始前，藻类细胞被驯化至特定的磷浓度。使用化学平衡软件MINEQL+ 4.61来计算锌离子（Zn²⁺）浓度。驯化后，将藻类细胞接种到含有不同锌浓度（0.09×10⁻⁶ mol L⁻¹至9.08×10⁻⁶ mol L⁻¹）的培养基中。研究表明，除了藻类生长速率降低外，磷对锌对微藻的毒性有重要影响。对于在磷浓度为2.3×10⁻⁴ mol L⁻¹、2.3×10⁻⁶ mol L⁻¹和1.0×10⁻⁶ mol L⁻¹下驯化的微藻，小头莱茵衣藻的抑制性锌离子浓度值分别为2.74×10⁻⁶ mol L⁻¹、0.58×10⁻⁶ mol L⁻¹和0.24×10⁻⁶ mol L⁻¹。生态毒理学研究应考虑金属浓度与不同磷值之间的相互作用，以提供关于浮游植物群落在环境中实际情况的现实数据。
