West of Scotland Specialist Virology Centre, Glasgow Royal Infirmary, Glasgow, UK.
West of Scotland Specialist Virology Centre, Glasgow Royal Infirmary, Glasgow, UK.
Diagn Microbiol Infect Dis. 2021 Feb;99(2):115221. doi: 10.1016/j.diagmicrobio.2020.115221. Epub 2020 Sep 21.
This study compares the performance of a commercial polymerase chain reaction (PCR) assay for detection of herpes simplex virus (HSV) 1 and 2, varicella zoster virus (VZV), and Treponema pallidum with laboratory-developed assays. A panel of 250 samples, previously tested using in-house assays, was tested on the PlexPCR® VHS assay. The panel consisted of 202 positive specimens [HSV-1 (n=51); HSV-2 (n=51); VZV (n=51); T. pallidum (n=49)] and 48 negative specimens. Genital samples had been previously tested for HSV-1/2 and T. pallidum and nongenital or unspecified samples for HSV-1/2 and VZV. The overall agreement between the PlexPCR® VHS and in-house assays was 97%. Negative agreement was ≥99%, and positive agreement for individual targets was 96% (47/49) for T. pallidum, 98% for HSV-1 and HSV-2 (50/51), and 100% (51/51) for VZV. Adoption of this assay would allow greater availability of molecular syphilis detection and enhance the diagnostic yield of samples collected from cutaneous/mucocutaneous lesions.
本研究比较了一种商用聚合酶链反应（PCR）检测试剂盒，用于检测单纯疱疹病毒（HSV）1 和 2、水痘带状疱疹病毒（VZV）和梅毒螺旋体，以及实验室开发的检测方法。一个由 250 个样本组成的小组，之前使用内部检测方法进行了测试，在 PlexPCR® VHS 检测试剂盒上进行了测试。该小组包括 202 个阳性样本[HSV-1（n=51）；HSV-2（n=51）；VZV（n=51）；T. pallidum（n=49）]和 48 个阴性样本。生殖器样本之前已经过 HSV-1/2 和 T. pallidum 的检测，非生殖器或未指定样本则进行 HSV-1/2 和 VZV 的检测。PlexPCR® VHS 和内部检测方法之间的总体一致性为 97%。阴性一致性≥99%，对于个别目标的阳性一致性为梅毒螺旋体 96%（47/49），HSV-1 和 HSV-2 为 98%（50/51），VZV 为 100%（51/51）。采用这种检测方法将能够更广泛地提供分子梅毒检测，并提高从皮肤/粘膜病变采集的样本的诊断产量。
