采用PlexPCR VHS检测法对梅毒确诊患者生殖器及生殖器外病变进行分子特征分析。
Molecular Characterization of Genital and Extragenital Lesions With the PlexPCR VHS Assay in Patients Diagnosed With Syphilis.
作者信息
Eguiluz Maria, Qquellon Jazmin, Vargas Silver K, Reyes-Diaz Michael, Konda Kelika A, Caceres Carlos F, Klausner Jeffrey D
机构信息
Center for Interdisciplinary Studies in Sexuality, AIDS and Society, Universidad Peruana Cayetano Heredia, Lima, Peru.
School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Peru.
出版信息
Open Forum Infect Dis. 2023 Sep 26;10(10):ofad483. doi: 10.1093/ofid/ofad483. eCollection 2023 Oct.
BACKGROUND
Syphilis diagnosis relies on immunologic markers and clinical protocols. However, syphilitic lesions can be confused with other genital ulcer diseases.
METHODS
Using a PlexPCR VHS assay, we analyzed lesion DNA samples from 87 individuals who were clinically diagnosed with early syphilis infection and had at least 1 positive serologic test result. DNA was detected by the PlexPCR VHS multiplex assay and β-globin genes.
RESULTS
Among the participants, 99% (86/87) had a positive rapid treponemal test result. DNA was successfully detected in 91% (79/87) of the lesion samples. PlexPCR VHS identified 5 herpes simplex virus (HSV)/ coinfections (2 HSV-1 and 3 HSV-2), only DNA in 62% (49/79), and only HSV-2 in 12.7% (10/79). While 19% (15/79) were negative for all pathogens, none were varicella zoster virus positive. The PlexPCR VHS had 68.4% agreement with the clinical diagnosis.
CONCLUSIONS
Since the PlexPCR VHS detects multiple organisms simultaneously, it can help to confirm actual syphilis and identify other pathogen coinfections or the pathogen causing the ulcer.
背景
梅毒诊断依赖于免疫标志物和临床方案。然而，梅毒病变可能与其他生殖器溃疡疾病相混淆。
方法
我们使用PlexPCR VHS检测法，分析了87例临床诊断为早期梅毒感染且至少有1次血清学检测结果呈阳性的个体的病变DNA样本。通过PlexPCR VHS多重检测法和β-珠蛋白基因检测DNA。
结果
在参与者中，99%（86/87）的快速梅毒螺旋体检测结果呈阳性。91%（79/87）的病变样本中成功检测到DNA。PlexPCR VHS检测出5例单纯疱疹病毒（HSV）/合并感染（其中2例为HSV-1，3例为HSV-2），仅62%（49/79）检测到DNA，仅12.7%（10/79）检测到HSV-2。虽然19%（15/79）的样本对所有病原体检测均为阴性，但无一例水痘带状疱疹病毒呈阳性。PlexPCR VHS与临床诊断的一致性为68.4%。
结论
由于PlexPCR VHS能同时检测多种病原体，它有助于确诊实际的梅毒感染，并识别其他病原体合并感染情况或导致溃疡的病原体。
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