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[用超速离心法分离沙门氏菌鞭毛蛋白抗原及其在制备红细胞H诊断剂中的应用]

[Isolation of flagellin antigens of salmonellae by the method of ultracentrifugation and their use for preparation of erythrocytic H-diagnostic agents].

作者信息

Rukhadze E Z, Levenson V I, Subbotina Iu L, Trushina V V, Kapuka L P

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1977 May(5):46-50.

PMID:331767
Abstract

Flagellar preparations were obtained from 6 salmonellae strains by differential ultracentrifugation; they were characterized by morphological, immunochemical, and serological tests. The results of investigations showed that the preparations possessed high serological activity; somatic antigen admixture was insignificant. Flagellins extracted from the flagellae were used to prepare erythrocytic H-diagnostic agents. The results of their use in the examination of sera of healthy persons, and of those suffering from typhoid fever and salmonellosis indicated the expediency of using passive H-hemagglutination for diagnostic purposes.

摘要

通过差速超速离心法从6株沙门氏菌中获得鞭毛制剂;通过形态学、免疫化学和血清学试验对其进行表征。研究结果表明,这些制剂具有高血清学活性;菌体抗原的混合量微不足道。从鞭毛中提取的鞭毛蛋白用于制备红细胞H诊断剂。将其用于检测健康人、伤寒患者和沙门氏菌病患者血清的结果表明,采用被动H血凝反应进行诊断是可行的。

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