*Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad, India; †Manipal Academy of Higher Education, Manipal, India; ‡The Centre of Excellence for Rare Eye Diseases, LV Prasad Eye Institute, Hyderabad, India; §The Cornea Institute, LV Prasad Eye Institute, Hyderabad, India; and ¶Jasti V Ramanamma Children's Eye Care Center, L V Prasad Eye Institute, Hyderabad, India.
Cornea. 2021 Apr;40(4):491-496. doi: 10.1097/ICO.0000000000002568.
The aim of this study is to determine the presence of oxidative stress markers in the aqueous humor (AH) and corneal tissues of patients with congenital hereditary endothelial dystrophy (CHED).
Interventional prospective study was undertaken to quantify levels of ascorbic acid and glutathione in the AH of patients with CHED. AH was collected from patients undergoing keratoplasty and levels of ascorbic acid and glutathione were determined using biochemical assays and measured spectrophotometrically. AH collected from pediatric patients with cataract were used as control. Corneal sections of patients who underwent penetrating keratoplasty were obtained, and presence of glutathione peroxidase 1, catalase, and superoxide dismutase was determined by immunohistochemistry. Tissue sections obtained from cadaveric corneas unsuitable for clinical transplant were used as control.
Significantly increased ascorbic acid levels were determined in patients with CHED (605.6 ± 158.9 μM) compared with those in controls (190.5 ± 74.72 μM). However, a trend toward reduced level of glutathione was detected in patients with CHED compared with that in the controls. Increased glutathione peroxidase 1 staining and reduced expression of catalase was detected in corneal tissues of patients with CHED compared with those in control corneal tissues. There was no apparent changes observed in the expression of superoxide dismutase in the corneal sections obtained from patients with CHED.
To the best of our knowledge, this is the first study to determine the levels of ascorbic acid and glutathione in AH of patients with CHED. Our data suggest the presence of oxidative stress in CHED that might be responsible for the pathological changes in patients with CHED.
本研究旨在确定先天性遗传性内皮层营养不良（CHED）患者房水中和角膜组织中氧化应激标志物的存在情况。
采用干预性前瞻性研究，定量检测 CHED 患者房水中抗坏血酸和谷胱甘肽的水平。从接受角膜移植术的患者中采集房水，并使用生化分析和分光光度法测定抗坏血酸和谷胱甘肽的水平。将从行白内障手术的儿科患者中采集的房水作为对照。获得行穿透性角膜移植术患者的角膜切片，并通过免疫组织化学法测定谷胱甘肽过氧化物酶 1、过氧化氢酶和超氧化物歧化酶的存在情况。将不适合临床移植的尸体角膜获得的组织切片作为对照。
与对照组（190.5±74.72μM）相比，CHED 患者的抗坏血酸水平显著升高（605.6±158.9μM）。然而，与对照组相比，CHED 患者的谷胱甘肽水平呈下降趋势。与对照组的角膜组织相比，CHED 患者的角膜组织中检测到谷胱甘肽过氧化物酶 1染色增加和过氧化氢酶表达减少。在从 CHED 患者获得的角膜切片中，超氧化物歧化酶的表达没有明显变化。
据我们所知，这是首次测定 CHED 患者房水中抗坏血酸和谷胱甘肽水平的研究。我们的数据表明，CHED 中存在氧化应激，这可能是导致 CHED 患者发生病理变化的原因。
