伊朗呼吸道感染患者中与23S基因变异相关的大环内酯类耐药的分子检测与评估
Molecular Detection and Evaluation of MLـ Resistance Associated with Mutation in 23S Gene among Iranian Patients with Respiratory Infections.
作者信息
Pouladi Iman, Mirnejad Reza, Rostampur Susan, Viesy Soghra, Niakan Mohammad
机构信息
Department of Medical Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Molecular Biology Research Center, System biology and Poisoning institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
出版信息
Rep Biochem Mol Biol. 2020 Jul;9(2):223-229. doi: 10.29252/rbmb.9.2.223.
BACKGROUND
is a common cause of community-acquired pneumonia. The global increased resistance of strains to macrolide (ML) has become a worrisome health problem. The widespread use of these medications has led to increased rate of reported ML-resistant (MRMP) throughout the world. This study was aimed to evaluate the resistance of against erythromycin due to mutations in the 23S rRNA gene of patients with respiratory infections in Iran.
METHODS
In this study, 100 samples of throat swab from a patient with respiratory problems were collected. After the cultured of all samples in -specific PPLO medium, PCR technique was performed with specific primers. Afterwards, the broth micro-dilution MIC assay was employed. Finally, the PCR product of the 23S gene was sequenced to detect mutations of domain V in 23S rRNA gene of MRMP.
RESULTS
It was found that 17 cases (17%) were positive for mycoplasma genus and six cases (6%) positive for species. Also, analysis of the sequence of 23S rRNA gene, revealed that one of the samples had mutations at positions A2431G and G2491A. All positive samples with 23S rRNA gene were sensitive to erythromycin.
CONCLUSION
These use of these antibiotics should be limited to prevent the emergence of MRMP in Iran.
背景
是社区获得性肺炎的常见病因。全球范围内菌株对大环内酯类（ML）的耐药性增加已成为一个令人担忧的健康问题。这些药物的广泛使用导致全球范围内大环内酯类耐药（MRMP）的报告率上升。本研究旨在评估伊朗呼吸道感染患者23S rRNA基因突变导致的对红霉素的耐药性。
方法
在本研究中，收集了100例呼吸道疾病患者的咽拭子样本。在特定的PPLO培养基中对所有样本进行培养后，使用特异性引物进行PCR技术。之后，采用肉汤微量稀释MIC测定法。最后，对23S基因的PCR产物进行测序，以检测MRMP的23S rRNA基因V结构域的突变。
结果
发现17例（17%）支原体属阳性，6例（6%）种阳性。此外，对23S rRNA基因序列的分析表明，其中一个样本在A2431G和G2491A位置发生了突变。所有23S rRNA基因阳性样本对红霉素敏感。
结论
在伊朗应限制这些抗生素的使用，以防止MRMP的出现。
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