Detection and Evaluation of Macrolide Resistance (Erythromycin) in hominis Isolated from Endocervical Specimens of Patients Referring to Ibn Sina Infertility Treatment Centre, Tehran, Iran.

BACKGROUND

is an important cause of bacterial infections of the genital tract. Macrolides are the first selective agents used to treat mycoplasma infections. However, widespread use of macrolides has led to a rapid and global emergence of macrolide-resistant strains. We evaluated macrolide resistance in isolated from endocervical specimens of patients who referred to Ibn Sina Infertility Centre in Tehran, Iran.

MATERIALS AND METHODS

In this cross-sectional descriptive-analytical study, 160 samples of Dacron endocervix swabs (80 infertile patient samples and 80 healthy controls) were collected and transferred to the laboratory. All samples were cultured in liquid pleuropneumonia-like organisms (PPLO) broth and PPLO agar solid media. After culturing and genome extraction, polymerase chain reaction (PCR) was performed using specific primers. Then, minimum inhibitory concentration (MIC) was obtained using the broth microdilution method. The MIC was recorded and reported for all samples positive for against erythromycin.

RESULTS

From the 160 endocervical specimens cultured in PPLO agar medium, 19 cases (23.75%) were positive. A total of 35 cases (42.5%) were positive using specific primers of species. MIC results from all samples positive for were measured against erythromycin. All of the samples were resistant to erythromycin.

CONCLUSION

The results of the present study showed that a significant percentage of infertile women were infected with . Also, MIC results from the broth microdilution method indicated that all strains positive for were also resistant to erythromycin.