Department of Histology Embriology, Afyonkarahisar Health Science University Faculty of Medicine, Afyonkarahisar, Turkey.
Department of Histology Embriology, Gaziantep Ünivercity Faculty of Medicine, Gaziantep, Turkey.
Mol Biol Rep. 2020 Dec;47(12):9313-9323. doi: 10.1007/s11033-020-05965-8. Epub 2020 Nov 12.
The current study investigated the change in umbilical cord tissue and the number of markers of Wharton's jelly mesenchymal stem cells (WJ-MSC) in pregnant women with gestational diabetes (GDM), with chronic diabetes who developed nephropathy as vascular complication (VC-PGDM), and healthy pregnant women as the control. The umbilical cords (UC) were investigated by the histomorphological method and the number of WJ-MSC were detected by flow-cytometry using the CD90, CD44, CD105, and CD73 markers in Wharton's jelly (WJ) isolated from fresh umbilical cords. The number of positive cells for CD 90, CD44, CD105, and CD73 were found to be elevated in the GDM group, whereas it was significantly diminished in the VC-PGDM group (p = 0.001, p = 0.001, p = 0.001, and p = 0.001). The only histopathological sign in the GDM group were an increased number of pores in the Wharton jelly. Artery wall thickness/cord diamater ratio was increased, which indicates an increase of the artery wall thickness in the VC- PGDM group (p = 0.039 and p = 0.048). The increase in umbilical cord diameter and number of Wharton jelly mesenchymal stem cells in babies of gestational diabetic mothers was considered as an effect of macrosomia seen in babies of mothers with gestational diabetes. Vasculopathy, a long-term complication of diabetes, is known to affect all tissues by causing marked lower perfusion and hypoxia, as well as a decrease in the MSC number in our study.
本研究调查了患有妊娠期糖尿病（GDM）的孕妇、患有并发血管病变的慢性糖尿病孕妇（VC-PGDM）和健康孕妇的脐带组织变化和华通氏胶间充质干细胞（WJ-MSC）标志物数量。通过组织形态学方法研究脐带，使用 CD90、CD44、CD105 和 CD73 标志物通过流式细胞术检测从新鲜脐带中分离的华通氏胶中的 WJ-MSC 数量。发现 GDM 组 CD90、CD44、CD105 和 CD73 的阳性细胞数量增加，而 VC-PGDM 组则显著减少（p=0.001、p=0.001、p=0.001 和 p=0.001）。GDM 组仅有的组织病理学特征是华通氏胶中孔的数量增加。动脉壁厚度/脐带直径比增加，这表明 VC-PGDM 组的动脉壁厚度增加（p=0.039 和 p=0.048）。患有妊娠期糖尿病的母亲的婴儿中，脐带直径和华通氏胶间充质干细胞数量的增加被认为是母亲患有妊娠期糖尿病时巨婴的影响。血管病变是糖尿病的长期并发症，已知会通过导致明显的低灌注和缺氧以及我们研究中 MSC 数量减少来影响所有组织。
