Center for Health & Bioresources, Molecular Diagnostics, Austrian Institute of Technology GmbH, Vienna, Austria.
Methods Mol Biol. 2021;2189:19-30. doi: 10.1007/978-1-0716-0822-7_2.
The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.
任何基于寡核苷酸的实验的成功都强烈依赖于对组成部分的精确设计。Oli2go 是一个用户友好的网络工具，它提供了高效的多重寡核苷酸设计，包括特异性和引物二聚体检查。它的全自动工作流程涉及使用特定参数来生成高质量寡核苷酸的重要设计步骤。本章描述了 oli2go 如何通过计算实现这些步骤。
