School of Biomedical Engineering, Capital Medical University, Beijing, China.
Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing, China.
Methods Mol Biol. 2021;2189:65-69. doi: 10.1007/978-1-0716-0822-7_5.
The CRISPR/Cas9 system has been developed as a powerful technology for both targeted genome editing and gene regulation. However, the design of efficient single-guide RNAs (sgRNAs) remains challenging with the consideration of many criteria. In this section, we introduce how to design sgRNA sequences and build genome-wide sgRNA library using CRISPR-ERA, which is one of the state-of-the-art designer webserver tools for sgRNA design based on a set of sgRNA design rules summarized from published reports.
CRISPR/Cas9 系统已被开发为一种强大的技术，可用于靶向基因组编辑和基因调控。然而，在考虑许多标准的情况下，设计高效的单指导 RNA（sgRNA）仍然具有挑战性。在本节中，我们介绍如何使用 CRISPR-ERA 设计 sgRNA 序列和构建全基因组 sgRNA 文库，CRISPR-ERA 是基于从已发表的报告中总结的一组 sgRNA 设计规则的最先进的 sgRNA 设计网络服务器工具之一。
