CRISPR-ERA：一个用于基因编辑和调控的向导 RNA 设计的网络服务器。

CRISPR-ERA: A Webserver for Guide RNA Design of Gene Editing and Regulation.

机构信息

School of Biomedical Engineering, Capital Medical University, Beijing, China.

Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing, China.

出版信息

Methods Mol Biol. 2021;2189:65-69. doi: 10.1007/978-1-0716-0822-7_5.

DOI:10.1007/978-1-0716-0822-7_5

PMID:33180293

Abstract

The CRISPR/Cas9 system has been developed as a powerful technology for both targeted genome editing and gene regulation. However, the design of efficient single-guide RNAs (sgRNAs) remains challenging with the consideration of many criteria. In this section, we introduce how to design sgRNA sequences and build genome-wide sgRNA library using CRISPR-ERA, which is one of the state-of-the-art designer webserver tools for sgRNA design based on a set of sgRNA design rules summarized from published reports.

摘要

CRISPR/Cas9 系统已被开发为一种强大的技术，可用于靶向基因组编辑和基因调控。然而，在考虑许多标准的情况下，设计高效的单指导 RNA（sgRNA）仍然具有挑战性。在本节中，我们介绍如何使用 CRISPR-ERA 设计 sgRNA 序列和构建全基因组 sgRNA 文库，CRISPR-ERA 是基于从已发表的报告中总结的一组 sgRNA 设计规则的最先进的 sgRNA 设计网络服务器工具之一。