Department of Physical Medicine ,Rheumatology & Rehabilitation, Assiut University Hospitals, Assiut, Egypt.
Department of Clinical Pathology, Assiut University Hospitals, Assiut, Egypt.
Egypt J Immunol. 2020 Jan;27(1):19-28.
Rheumatoid arthritis (RA) is one of the common autoimmune diseases, which affected by genetic and environmental factors. IL-12 is important cytokine that play an effective role in the inflammatory reaction of RA. It regulates the balance between Th1 and Th2 cells. Gene polymorphism of cytokines may predispose to susceptibility and severity of RA. To assess the association between single nucleotide polymorphism (SNP) in IL-12B gene (rs3212227 A/C) and serum level of IL-12 with the development and or activity of RA disease in Egyptian population. Sixty RA patients and thirty healthy individuals were studied for IL-12B gene (rs3212227 A/C) polymorphism using PCR-RFLP. Serum level of IL-12 was measured by ELISA. The frequency of genotype AC, CC, AC+CC and C allele were significantly higher in patients compared to control group (P < 0.02, 0.007, 0.02) respectively. Serum level of IL-12 was significantly higher in patients compared to control (P < 0.000). Patients who carry AC+CC genotypes had significantly higher DAS28, RF, ACCP and IL-12 compared to AA genotype patients (P < 0.05, 0.000, 0.000, 0.000) respectively. RA patients who carry AC, CC genotypes had more positive inflammatory markers (RF, ACCP) with P < 0.000, 0.05 respectively. Significant positive correlation was found between serum IL-12 and number of swollen joints, RF and ACCP. Present findings suggest that IL-12B gene (rs3212227 A/C) may be associated with development and activity of RA and that serum IL-12 can be used as predictor of activity of the disease.
类风湿关节炎（RA）是一种常见的自身免疫性疾病，受遗传和环境因素的影响。IL-12 是一种重要的细胞因子，在 RA 的炎症反应中发挥着有效的作用。它调节 Th1 和 Th2 细胞之间的平衡。细胞因子的基因多态性可能导致 RA 的易感性和严重程度。评估 IL-12B 基因（rs3212227 A/C）单核苷酸多态性与血清 IL-12 水平与埃及人群 RA 发病和/或疾病活动的相关性。使用 PCR-RFLP 研究了 60 例 RA 患者和 30 例健康个体的 IL-12B 基因（rs3212227 A/C）多态性。通过 ELISA 测量血清 IL-12 水平。与对照组相比，患者的基因型 AC、CC、AC+CC 和 C 等位基因的频率明显更高（P < 0.02、0.007、0.02）。与对照组相比，患者的血清 IL-12 水平明显更高（P < 0.000）。与 AA 基因型患者相比，携带 AC+CC 基因型的患者的 DAS28、RF、ACCP 和 IL-12 水平明显更高（P < 0.05、0.000、0.000、0.000）。携带 AC、CC 基因型的 RA 患者具有更多的阳性炎症标志物（RF、ACCP），P 值均 < 0.000、0.05。血清 IL-12 与肿胀关节数、RF 和 ACCP 之间存在显著正相关。本研究结果表明，IL-12B 基因（rs3212227 A/C）可能与 RA 的发生和活动有关，血清 IL-12 可作为疾病活动的预测指标。
