Department of Medical Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Egypt J Immunol. 2020 Jan;27(1):29-36.
Type 1 diabetes mellitus (T1DM) remains the most common form of diabetes in childhood. The incidence of type 1 diabetes is continuously increased. Zinc transporter protein 8 antibodies (ZnT8A) measurement can be helpful in detection of suspected new cases of type 1diabetes when other islet auto antibodies are negative. We evaluated the role of ZnT8A in diagnosis of new cases of T1DM in comparison to islet cell antibody (ICA), and assessed its prediction value among siblings. 31 of newly diagnosed T1DM patients and 55 age and sex matched healthy siblings were included. Measurements of ZnT8A and ICA was carried out by ELISA. ZnT8A had 45% sensitivity and 69% specificity while ICA had 64.5% sensitivity and 83.64% specificity. 22.6% of diabetic patients had high level of ZnT8A as compared to 20% of siblings (P < 0.001 and P < 0.001, respectively). 28.6% of diabetic patients with high titer ZnT8A had positive ICA (P < 0.04) as compared to 63.6% in sibling group (P < 0.001). It is concluded that ZnT8A and ICA play an important role in diagnosis and prediction of T1DM cases.
1 型糖尿病（T1DM）仍然是儿童中最常见的糖尿病形式。1 型糖尿病的发病率持续增加。当其他胰岛自身抗体阴性时，锌转运蛋白 8 抗体（ZnT8A）的测量有助于检测疑似 1 型糖尿病的新病例。我们评估了 ZnT8A 在诊断新诊断的 1 型糖尿病病例中的作用，与胰岛细胞抗体（ICA）进行比较，并评估了其在兄弟姐妹中的预测价值。纳入 31 名新诊断的 1 型糖尿病患者和 55 名年龄和性别匹配的健康兄弟姐妹。通过 ELISA 进行 ZnT8A 和 ICA 的测量。ZnT8A 的灵敏度为 45%，特异性为 69%，而 ICA 的灵敏度为 64.5%，特异性为 83.64%。与 20%的兄弟姐妹相比，22.6%的糖尿病患者具有高水平的 ZnT8A（P<0.001 和 P<0.001）。与兄弟姐妹组的 63.6%相比，28.6%高滴度 ZnT8A 的糖尿病患者具有阳性 ICA（P<0.04）。结论：ZnT8A 和 ICA 在 1 型糖尿病病例的诊断和预测中发挥重要作用。
