State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Plant Sci. 2020 Nov;300:110640. doi: 10.1016/j.plantsci.2020.110640. Epub 2020 Aug 22.
Cucumber (Cucumis sativus) is one of the most widely cultivated vegetable crops in the world, and its yield is often reduced due to the infection of Botrytis cinerea (B. cinerea), which causes a serious disease. However, few genes involved in the response to B. cinerea have been identified in cucumber. In this study, we identified that CsWRKY10 plays a key role in the cucumber resistance to B. cinerea because that the overexpression of CsWRKY10 significantly increased the susceptibility to B. cinerea in cucumber. After the pathogen infection, the enzyme activities of catalase, superoxide dismutase and peroxidase in transgenic plants were affected, resulting in the decrease in reactive oxygen species (ROS) contents. In addition, the light microscopic images showed that overexpression of CsWRKY10 promoted the spore germination and mycelia elongation of B. cinerea in cucumber. Importantly, after B. cinerea infection, the contents of jasmonic acid (JA) are decreased, and the expression levels of JA- and salicylic acid- related defence genes significantly changed in transgenic plants. In contrast, overexpression of CsWRKY10 enhanced resistance to Corynespora cassiicola in cucumber. Collectively, this study indicated that CsWRKY10 negatively regulates the resistance of cucumber to B. cinerea by reducing the ROS contents and inhibiting the JA-mediated resistance signalling pathway, but strengthens resistance to Corynespora cassiicola.
黄瓜（Cucumis sativus）是世界上种植最广泛的蔬菜作物之一，但其产量常因感染灰葡萄孢（Botrytis cinerea，B. cinerea）而降低，导致严重的疾病。然而，在黄瓜中，参与对灰葡萄孢反应的基因很少被鉴定出来。在本研究中，我们鉴定出 CsWRKY10 在黄瓜对灰葡萄孢的抗性中起着关键作用，因为 CsWRKY10 的过表达显著增加了黄瓜对灰葡萄孢的敏感性。在病原体感染后，转基因植物中过氧化氢酶、超氧化物歧化酶和过氧化物酶的酶活性受到影响，导致活性氧（ROS）含量下降。此外，光镜图像显示，CsWRKY10 的过表达促进了灰葡萄孢在黄瓜中的孢子萌发和菌丝伸长。重要的是，在灰葡萄孢感染后，茉莉酸（JA）的含量降低，并且与 JA 和水杨酸相关的防御基因的表达水平在转基因植物中显著改变。相比之下，CsWRKY10 的过表达增强了黄瓜对尾孢菌的抗性。总的来说，这项研究表明，CsWRKY10 通过降低 ROS 含量和抑制 JA 介导的抗性信号通路来负调控黄瓜对灰葡萄孢的抗性，但增强了对尾孢菌的抗性。
