Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA.
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York City, NY.
Appl Immunohistochem Mol Morphol. 2021 Mar 1;29(3):175-179. doi: 10.1097/PAI.0000000000000882.
Intrahepatic cholangiocarcinomas (ICCs) show morphologic diversity, ranging from tumors composed of nonmucinous small ducts to mucin-producing large duct tumors to tumors with mixed hepatocellular carcinoma features. Diagnosing ICCs can be difficult, especially on biopsy, not only because of the morphologic diversity, but also because metastatic tumors are often in the differential diagnosis. Recently, branched DNA-based albumin RNA in situ hybridization (ISH) has been shown to be a potential sensitive and specific marker for ICC with 99% sensitivity. Using a different RNA ISH technology, we evaluated the expression of albumin RNA ISH in ICC. We performed RNA ISH for albumin using RNAscope on 43 ICCs in a triplicate tissue microarray. Albumin RNA ISH was positive in 18 of 43 (42%) ICCs. Five of the 6 (83%) combined hepatocellular carcinoma-CC were positive in the CC component. None of the tumors with mucin production were positive (0/9). In our cohort, albumin RNA ISH showed a sensitivity of 42% in ICCs, supporting the morphologic diversity of ICCs. Albumin RNA ISH does not appear to be a highly sensitive marker for ICC and hence cannot be used as a stand-alone marker for ICC.
肝内胆管细胞癌 (ICC) 表现出形态学多样性，从由非黏液性小胆管组成的肿瘤到产生黏液的大胆管肿瘤，再到具有混合肝细胞癌特征的肿瘤。ICC 的诊断可能具有挑战性，特别是在活检时，不仅因为形态学的多样性，还因为转移性肿瘤通常也在鉴别诊断中。最近，基于分支 DNA 的白蛋白 RNA 原位杂交 (ISH) 已被证明是 ICC 的一种潜在敏感和特异的标志物，其敏感性为 99%。我们使用不同的 RNA ISH 技术评估了 ICC 中白蛋白 RNA ISH 的表达。我们在一个三重复组织微阵列中对 43 个 ICC 进行了 RNAscope 白蛋白 RNA ISH 检测。43 个 ICC 中有 18 个（42%）呈白蛋白 RNA ISH 阳性。6 个混合性肝细胞癌-ICC 中有 5 个（83%）在 CC 成分中呈阳性。没有一个黏液产生的肿瘤呈阳性（0/9）。在我们的队列中，ICC 中白蛋白 RNA ISH 的敏感性为 42%，支持 ICC 的形态学多样性。白蛋白 RNA ISH 似乎不是 ICC 的高度敏感标志物，因此不能作为 ICC 的单一标志物。
