Hernández-Bautista Liz Mariana, Márquez-Preciado Raúl, Ortiz-Magdaleno Marine, Pozos-Guillén Amaury, Aranda-Romo Saray, Sánchez-Vargas Luis Octavio
J Clin Pediatr Dent. 2020 Sep 1;44(5):315-322. doi: 10.17796/1053-4625-44.5.5.
The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans.
The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours. Serial dilutions of 10-1 to 10-7 were made and viability was verified and quantified. For the antagonism tests, a suspension/inoculum of Lactobacillus strains recovered from each commercial preparation (4-30 × 109) and C. albicans ATCC 90028 (1.5-8 × 108) was prepared in MRS broth and incubated for 48 hours at 36°C, then plated on Dextrose Sabouraud Agar with Chloramphenicol and Rogosa Agar and the colony-forming units (CFU) were quantified. Additionally, viability was evaluated using the LIVE/DEAD® Yeast and Bacterial Viability kit.
The probiotic that produced the highest acidity of the medium was Lactipan®, followed by Probiseis® and Liolactil®, while Probio Hp+® showed the least change. Probiseis® was determined to have the highest growth of probiotic bacteria and the highest inhibition on C. albicans, followed by Lactipan®; Liolactil® and ProbioHp+® showed the least effect. In fluorescence tests, ProBiseis® showed the best effect, followed by Liolactil® and Lactipan®; Probio Hp+® had less of an effect.
Two commercial products (ProBiseis and Lactipan) whose formulations have L. acidophilus, L. casei, L. rhamnosus, L. plantarum, B. infantis, and S. thermophilus have a greater inhibitory effect on C. albicans ATCC 90028.
评估从五种商业益生菌中分离出的乳酸杆菌和双歧杆菌对白色念珠菌生长的拮抗作用。
从五种商业益生菌中分离并培养乳酸杆菌和双歧杆菌菌株：Probio Hp+®、ProBiseis®、Lactipan®、Liolactil®和Lacteol Fort®；每种取50毫克进行水化处理，在乳酸杆菌MRS（德氏、罗格斯和夏普）肉汤中培养，于37°C搅拌（120转/分钟）孵育24小时。进行10⁻¹至10⁻⁷的系列稀释，并验证和量化活力。对于拮抗试验，在MRS肉汤中制备从每种商业制剂中分离出的乳酸杆菌菌株悬液/接种物（4 - 30×10⁹）和白色念珠菌ATCC 90028（1.5 - 8×10⁸），于36°C孵育48小时，然后接种到含氯霉素的葡萄糖沙氏琼脂和罗格斯琼脂上，对菌落形成单位（CFU）进行量化。此外，使用LIVE/DEAD®酵母和细菌活力试剂盒评估活力。
使培养基酸度最高的益生菌是Lactipan®，其次是ProBiseis®和Liolactil®，而Probio Hp+®的变化最小。确定ProBiseis®的益生菌生长最高，对白色念珠菌的抑制作用最强，其次是Lactipan®；Liolactil®和ProbioHp+®的作用最小。在荧光试验中，ProBiseis®效果最佳，其次是Liolactil®和Lactipan®；Probio Hp+®的效果较差。
两种商业产品（ProBiseis和Lactipan），其配方含有嗜酸乳杆菌、干酪乳杆菌、鼠李糖乳杆菌、植物乳杆菌、婴儿双歧杆菌和嗜热链球菌，对白色念珠菌ATCC 90028具有更大的抑制作用。
