Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China.
Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China.
Carbohydr Polym. 2021 Jan 15;252:117186. doi: 10.1016/j.carbpol.2020.117186. Epub 2020 Oct 4.
Pomelo fruitlets contain various active substances that are easily collected and processed. Here, the biological effects of pomelo fruitlet dietary fiber were investigated in vivo and in vitro. Total dietary fiber (TDF), soluble dietary fiber (SDF), and insoluble dietary fiber (IDF) values of pomelo fruitlets were 75.64 ± 3.65 %, 10.10 ± 1.39 %, and 62.48 ± 3.68 %, respectively. The main monosaccharides identified were rhamnose, arabinose, galactose, and glucose. All fibers scavenged free 2,2'-diphenyl-1-picrylhydrazyl radicals and reduced ferric cations. The water-holding, oil-holding, and swelling capacities of the fibers retarded glucose diffusion, inhibited α-amylase, and influenced cholesterol micelle formation. In a mouse model of alloxan-induced diabetes, SDF improved glucose tolerance, controlled blood glucose, and reduced serum insulin better than TDF or IDF. All fiber types decreased the total cholesterol content and the prevalence of Bacteroidetes, Proteobacteria, and Ruminococcaceae, but increased the abundance of Firmicutes, Lactobacillus, and Prevotellaceae in hyperglycemic mice.
柚子果实含有多种易于采集和加工的活性物质。本研究旨在体内和体外探究柚子果实膳食纤维的生物学效应。柚子果实的总膳食纤维（TDF）、可溶性膳食纤维（SDF）和不溶性膳食纤维（IDF）含量分别为 75.64±3.65%、10.10±1.39%和 62.48±3.68%。鉴定出的主要单糖为鼠李糖、阿拉伯糖、半乳糖和葡萄糖。所有膳食纤维均能清除 2,2'-二苯基-1-苦基肼自由基和还原铁离子。持水力、持油力和溶胀能力均能延缓葡萄糖扩散，抑制α-淀粉酶，并影响胆固醇胶束形成。在链脲佐菌素诱导的糖尿病小鼠模型中，SDF 改善葡萄糖耐量、控制血糖和降低血清胰岛素的效果优于 TDF 或 IDF。所有膳食纤维类型均降低了总胆固醇含量和拟杆菌门、变形菌门和瘤胃球菌科的流行率，但增加了高血糖小鼠厚壁菌门、乳杆菌属和普雷沃氏菌科的丰度。
