R Powley Charles, Dong Jinlan, Hannas Bethany R, Shen Zhihua Amanda
R&D Center, Corteva Agriscience, PO Box 30, 1090 Elkton Rd., Bldg. 320, Newark, DE 19714, USA.
Center for PFAS Solutions, 272 Quigley Blvd., New Castle, DE 19720, USA.
Bioanalysis. 2020 Dec;12(23):1689-1698. doi: 10.4155/bio-2020-0248. Epub 2020 Nov 13.
Numerous guideline studies required for regulatory toxicity testing now include the measurement of the thyroid hormones 3,3',5-triiodo-L-thyronine (T3) and L-thyroxine (T4) in blood serum from rodents. A rapid, high-throughput method for the determination of the thyroid hormones T4 and T3 is reported. Sample preparation is done using a 96-well microtiter plate format. Stable isotope analogs of both hormones are used as internal standards for study and quality control samples. The validated quantification levels are T3: 10 pg/ml and T4: 1 ng/ml, with CVs of <10% at the limit of quantification and up to 50*limit of quantification. The use of isotope analog internal standards eliminates the need for quantitative transfers and complete evaporations.
目前，监管毒性测试所需的众多指南研究包括测量啮齿动物血清中的甲状腺激素3,3',5-三碘-L-甲状腺原氨酸（T3）和L-甲状腺素（T4）。本文报道了一种快速、高通量测定甲状腺激素T4和T3的方法。样品制备采用96孔微量滴定板形式。两种激素的稳定同位素类似物用作研究样品和质量控制样品的内标。验证后的定量限为T3：10 pg/ml，T4：1 ng/ml，在定量限处变异系数<10%，最高可达定量限的50倍。使用同位素类似物内标无需进行定量转移和完全蒸发。
