鉴定 EL-4 肿瘤中 CD137 和 CD137L 表达的细胞。

Identification of CD137- and CD137L-Expressing Cells in EL-4 Tumor.

机构信息

School of Biological Sciences, University of Ulsan, Ulsan, Republic of Korea.

Biomedical Research Center, Ulsan University Hospital, College of Medicine, University of Ulsan, Ulsan, Republic of Korea.

出版信息

Methods Mol Biol. 2021;2248:221-229. doi: 10.1007/978-1-0716-1130-2_16.

DOI:10.1007/978-1-0716-1130-2_16

PMID:33185879

Abstract

The tumor microenvironment (TME) contains noncancerous cells such as various types of immune cells and fibroblasts. Cancer cells direct these stromal cells to create a microenvironment favorable for tumor growth and intercellular interactions have a critical role in this process. In established tumors, interactions between CD137 and its ligand (CD137L) contribute to tumor immune evasion and tumor growth. Therefore, it is important to identify cells expressing CD137 and CD137L within tumors. In this chapter, we will introduce a simple, powerful method of analyzing CD137- and CD137L-expressing tumor cells using Fluorescence-activated cell sorting.

摘要

肿瘤微环境 (TME) 包含非癌细胞，如各种类型的免疫细胞和成纤维细胞。癌细胞指导这些基质细胞创造有利于肿瘤生长的微环境，细胞间相互作用在这个过程中起着关键作用。在已建立的肿瘤中，CD137 与其配体（CD137L）之间的相互作用有助于肿瘤免疫逃逸和肿瘤生长。因此，识别肿瘤内表达 CD137 和 CD137L 的细胞非常重要。在本章中，我们将介绍一种简单而强大的方法，使用荧光激活细胞分选来分析表达 CD137 和 CD137L 的肿瘤细胞。