Division of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India.
Methods Mol Biol. 2021;2248:231-241. doi: 10.1007/978-1-0716-1130-2_17.
The tumor necrosis factor (TNF) superfamily (TNFSF) members play crucial roles in the pathogenesis of acute and chronic kidney diseases. They orchestrate inflammation, cell survival, tissue repair as well as fibrosis in kidneys upon injury by engaging respective receptors on the cell membranes. Therefore, the TNFSF ligands, as well as their receptors, have gained enormous interest as putative drug targets to combat kidney diseases. It was shown that the expression profiles of TNFSF ligands differ in human and mice solid organs, as well as during acute kidney injuries and chronic kidney diseases in mice. This indicates that the mRNA expressions of TNFSF ligands highly depend on the species and nature of the injury, which needs to be given appropriate consideration while extrapolating the data between species and between different kidney diseases. The protocol presented here describes the use of real-time polymerase chain reaction (RT-PCR) to quantify the mRNA expressions of TNFSF ligands in healthy and injured murine kidneys.
肿瘤坏死因子（TNF）超家族（TNFSF）成员在急性和慢性肾脏疾病的发病机制中起着至关重要的作用。它们通过在细胞膜上结合各自的受体，在肾脏受到损伤时协调炎症、细胞存活、组织修复以及纤维化。因此，TNFSF 配体及其受体作为潜在的药物靶点，以对抗肾脏疾病引起了极大的关注。已经表明，TNFSF 配体的表达谱在人和小鼠的实体器官中以及在小鼠的急性肾损伤和慢性肾脏疾病中有所不同。这表明 TNFSF 配体的 mRNA 表达高度取决于物种和损伤的性质，在物种间和不同肾脏疾病间推断数据时需要给予适当考虑。本方案介绍了使用实时聚合酶链反应（RT-PCR）定量测定健康和受损小鼠肾脏中 TNFSF 配体的 mRNA 表达。
