Soma G, Kitahara N, Tsuji Y, Kato M, Oshima H, Gatanaga T, Inagawa H, Noguchi K, Tanabe Y, Mizuno D
Biotechnology Research Center, Teikyo University, Kanagawa, Japan.
Biochem Biophys Res Commun. 1987 Oct 29;148(2):629-35. doi: 10.1016/0006-291x(87)90923-5.
Two new recombinant TNFs (named rTNF-Scw1 and -Scw2) with higher basicity than conventional recombinant human TNF-alpha (rTNF-alpha) in the N-terminal region were constructed. Their sequences were constructed based on those of partially purified cytotoxic factors from the culture supernatant of acute monocytic leukemia cells THP-1, which unlike rTNF-alpha are cytotoxic to T24 bladder carcinoma cells in vitro. These new rTNF-Ss showed a broader cytotoxicity to tumor cells than rTNF-alpha. This increase in the basicity of the N-terminal region over that of conventional TNF significantly increased the cytotoxicity on tumor cells in vivo as well as in vitro.
构建了两种新的重组肿瘤坏死因子（分别命名为rTNF-Scw1和-Scw2），其N端区域的碱性高于传统重组人肿瘤坏死因子-α（rTNF-α）。它们的序列是基于急性单核细胞白血病细胞THP-1培养上清液中部分纯化的细胞毒性因子构建的，与rTNF-α不同，这些细胞毒性因子在体外对T24膀胱癌细胞具有细胞毒性。这些新的rTNF-Ss对肿瘤细胞的细胞毒性比rTNF-α更广。与传统肿瘤坏死因子相比，N端区域碱性的增加显著提高了其在体内和体外对肿瘤细胞的细胞毒性。
