An endoplasmic reticulum targetable turn-on fluorescence probe for imaging application of carbon monoxide in living cells.

Carbon monoxide (CO) is a significant mediator in regulating endoplasmic reticulum (ER) stress, and its level may play a potential role in the treatment of vascular diseases combined with ER stress. In-situ visualization of CO in the ER helps to elucidate its physiological and pathological mechanistic behavior. Herein, a novel CO fluorescent probe (Na-CM-ER) with ER-targeting characteristics was structured. Na-CM-ER with naphthalimide as a fluorescent group, under the trigger of CO, an ICT (Intramolecular Charge Transfer) mechanism was constructed by converting a nitro group to an amino group and showed dazzling green fluorescence. Na-CM-ER exhibited satisfactory response speed, selectivity, photo-stability and sensitivity to CO in vitro. Furthermore, biological imaging experiments demonstrated that Na-CM-ER could monitor the changes of exogenous/endogenous CO in living cells and possess an ER-targeting property. To sum up, we hope that Na-CM-ER can be as a serviceable molecular tool for imaging CO in cellular ER.