Zhang Yunyan, Tang Yonghe, Kong Xiuqi, Lin Weiying
Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119150. doi: 10.1016/j.saa.2020.119150. Epub 2020 Nov 4.
Carbon monoxide (CO) is a significant mediator in regulating endoplasmic reticulum (ER) stress, and its level may play a potential role in the treatment of vascular diseases combined with ER stress. In-situ visualization of CO in the ER helps to elucidate its physiological and pathological mechanistic behavior. Herein, a novel CO fluorescent probe (Na-CM-ER) with ER-targeting characteristics was structured. Na-CM-ER with naphthalimide as a fluorescent group, under the trigger of CO, an ICT (Intramolecular Charge Transfer) mechanism was constructed by converting a nitro group to an amino group and showed dazzling green fluorescence. Na-CM-ER exhibited satisfactory response speed, selectivity, photo-stability and sensitivity to CO in vitro. Furthermore, biological imaging experiments demonstrated that Na-CM-ER could monitor the changes of exogenous/endogenous CO in living cells and possess an ER-targeting property. To sum up, we hope that Na-CM-ER can be as a serviceable molecular tool for imaging CO in cellular ER.
一氧化碳（CO）是调节内质网（ER）应激的重要介质，其水平可能在治疗伴有内质网应激的血管疾病中发挥潜在作用。在内质网中对CO进行原位可视化有助于阐明其生理和病理机制行为。在此，构建了一种具有内质网靶向特性的新型CO荧光探针（Na-CM-ER）。以萘酰亚胺为荧光基团的Na-CM-ER，在CO的触发下，通过将硝基转化为氨基构建了一种分子内电荷转移（ICT）机制，并呈现出耀眼的绿色荧光。Na-CM-ER在体外对CO表现出令人满意的响应速度、选择性、光稳定性和敏感性。此外，生物成像实验表明，Na-CM-ER可以监测活细胞中外源/内源CO的变化，并具有内质网靶向特性。综上所述，我们希望Na-CM-ER能够成为一种用于内质网中CO成像的有用分子工具。
