小肠刷状缘膜凝集素结合能力的定量测定。一种酶联凝集素吸附测定法（ELLSA）。

Quantitative determination of the lectin binding capacity of small intestinal brush-border membrane. An enzyme linked lectin sorbent assay (ELLSA).

作者信息

Hendriks H G, Koninkx J F, Draaijer M, van Dijk J E, Raaijmakers J A, Mouwen J M

机构信息

Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.

出版信息

Biochim Biophys Acta. 1987 Dec 11;905(2):371-5. doi: 10.1016/0005-2736(87)90465-2.

DOI:10.1016/0005-2736(87)90465-2

PMID:3318929

Abstract

A test to determine quantitatively the lectin binding sites in brush-border membranes has been developed. Highly purified bovine small intestinal brush-border membranes were prepared, and subsequently coated directly to the bottom of a microtiter plate. Soybean agglutinin conjugated with peroxidase was coupled to its binding sites in the brush-border membranes and the peroxidase activity was determined in a spectrophotometer. The number of soybean agglutinin binding sites in the brush-border membranes has been established by means of iterized computer fit analysis of the data, indicating values for maximal binding of 7.10(-7) M soybean agglutinin per mg of brush-border membrane protein and a dissociation constant of 1.5.10(-5) M.

摘要

已开发出一种用于定量测定刷状缘膜中凝集素结合位点的试验。制备了高度纯化的牛小肠刷状缘膜，随后直接包被到微量滴定板底部。将与过氧化物酶偶联的大豆凝集素与刷状缘膜中的结合位点结合，并在分光光度计中测定过氧化物酶活性。通过对数据进行迭代计算机拟合分析，确定了刷状缘膜中大豆凝集素结合位点的数量，结果表明每毫克刷状缘膜蛋白与大豆凝集素的最大结合值为7.10(-7) M，解离常数为1.5.10(-5) M。