School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.
School of Pharmacy, Chongqing University, Chongqing, 401331, China.
J Chromatogr A. 2020 Dec 20;1634:461675. doi: 10.1016/j.chroma.2020.461675. Epub 2020 Nov 2.
A novel multi-mode and chiral separation stationary phase co-modified with copolymer composed of N-isopropyl acrylamide (NIPAM) and aspartame was synthesized by atom transfer radical polymerization (ATRP) reaction. The synthetic material was evaluated using thermogravimetric analysis (TGA), Fourier transform infrared spectrometry (FT-IR) and elemental analysis (EA). Analytes including hydrophobic, hydrophilic, alkaline and acidic compounds were separated well using the prepared stationary phase named Sil-PPAM-NIPAM. Besides, the separation of chiral compounds proved that the developed column also has the potential of chiral separation ability. In summary, the prepared column possesses excellent hydrophilic interaction, ion exchange, reversed-phase and chiral separation modes during the separation of complex and chiral compounds.
一种新型的多模式和手性分离固定相，通过原子转移自由基聚合（ATRP）反应与由 N-异丙基丙烯酰胺（NIPAM）和阿斯巴甜组成的共聚物进行了共聚改性。使用热重分析（TGA）、傅里叶变换红外光谱（FT-IR）和元素分析（EA）对合成材料进行了评估。使用所制备的固定相Sil-PPAM-NIPAM 可以很好地分离包括疏水性、亲水性、碱性和酸性化合物在内的分析物。此外，手性化合物的分离证明了所开发的色谱柱也具有手性分离能力。综上所述，在分离复杂和手性化合物时，所制备的色谱柱具有出色的亲水相互作用、离子交换、反相和手性分离模式。
