TB Control Unit, Tan Tock Seng Hospital, Singapore, Singapore.
Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore.
Eur J Clin Microbiol Infect Dis. 2021 May;40(5):1079-1083. doi: 10.1007/s10096-020-04100-6. Epub 2020 Nov 15.
There were 290 multidrug-resistant (MDR)-TB cases diagnosed in Singapore from 2006 to 2018. Eighty-one percent were foreign-born. Spoligotyping and MIRU-VNTR methods identified 108 patients in 24 clusters. The Beijing spoligotype accounted for 22 clusters. Whole genome sequencing (WGS) analysis reduced the number of clustered patients and clusters to 43 and nine respectively. One MIRU cluster was redefined into three WGS clusters. All the clusters had foreign-born source cases. Forty percent of local-born, versus 9% of foreign-born, MDR-TB cases belonged to WGS clusters. WGS more accurately elucidated potential MDR-TB transmission which was overestimated by conventional genotyping methods in Singapore.
2006 年至 2018 年，新加坡共诊断出 290 例耐多药结核病（MDR-TB）病例。81%的病例为外国出生。 spoligotyping 和 MIRU-VNTR 方法鉴定出 24 个集群中的 108 名患者。北京 spoligotype 占 22 个集群。全基因组测序（WGS）分析将聚类患者和聚类数量分别减少到 43 个和 9 个。一个 MIRU 集群被重新定义为三个 WGS 集群。所有集群的源病例均为外国出生。40%的本地出生 MDR-TB 病例，而非 9%的外国出生 MDR-TB 病例，属于 WGS 集群。WGS 更准确地阐明了潜在的 MDR-TB 传播，而传统的基因分型方法在新加坡高估了这种传播。
