Chen Jiao, Lu Yixin, Huang Wen, Wu Juzhen, Li Binling, Zhang Jianqiang
School of Materials and Environmental Engineering, Chengdu Technological University, Chengdu, People's Republic of China.
Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, People's Republic of China.
Environ Technol. 2022 May;43(12):1770-1782. doi: 10.1080/09593330.2020.1850877. Epub 2020 Nov 26.
Anaerobic ammonia oxidation (anammox) process was achieved in a constructed rapid infiltration (CRI) system and the effect of organic matter on the anammox performance and microbial community structure was investigated. The results showed that the removal efficiencies of -N, N and TN were 99.7 ± 0.3%, 99.8 ± 0.2% and 91.3 ± 0.2% respectively after 83 days of acclimation without the presence of organic matter in the influent. The average TN removal efficiency increased by 3.2%-7.7% due to the synergistic effect of anammox and denitrification at a low level of organic matter concentration (10-30 mg COD/L). At medium or high organic matter concentration (50-100 mg COD/L), denitrification gradually replaced anammox as the predominant nitrogen removal route due to its stronger ability to compete with substrate, resulting in a significant decline in anammox activity. The contribution rate of anammox to nitrogen removal dropped by 70.3% with the influent COD increased from 0 to 100 mg/L, and the TN removal efficiency decreased to 68.4 ± 3.6% since the anammox was seriously suppressed. 16S rRNA high-throughput sequencing analysis illustrated that the genus was the predominant anammox bacteria (AAOB) with a relative abundance of 12.63% when no organic matter was applied. While the heterotrophic denitrifying bacteria (DNB) gradually dominated the community with the elevated organic matter introduction. The findings of this study provide useful information for the stable operation and optimal regulation of anammox in the CRI system when the influent contains organic matter.
在构建的快速渗滤（CRI）系统中实现了厌氧氨氧化（anammox）过程，并研究了有机物对anammox性能和微生物群落结构的影响。结果表明，在进水无有机物的情况下驯化83天后，-N、N和TN的去除效率分别为99.7±0.3%、99.8±0.2%和91.3±0.2%。在低有机物浓度（10-30mg COD/L）下，由于anammox和反硝化的协同作用，平均TN去除效率提高了3.2%-7.7%。在中高有机物浓度（50-100mg COD/L）下，由于反硝化与底物的竞争能力更强，反硝化逐渐取代anammox成为主要的脱氮途径，导致anammox活性显著下降。随着进水COD从0增加到100mg/L，anammox对氮去除的贡献率下降了70.3%，由于anammox受到严重抑制，TN去除效率降至68.4±3.6%。16S rRNA高通量测序分析表明，在不施加有机物时，属是主要的厌氧氨氧化细菌（AAOB），相对丰度为12.63%。而随着有机物引入量的增加，异养反硝化细菌（DNB）逐渐在群落中占主导地位。本研究结果为进水含有有机物时CRI系统中anammox的稳定运行和优化调控提供了有用信息。
