Purification and properties of human erythrocyte inosine triphosphate pyrophosphohydrolase.
作者信息
Vanderheiden B S
出版信息
J Cell Physiol. 1979 Jan;98(1):41-7. doi: 10.1002/jcp.1040980106.
PMID:33191
Abstract
Inosine triphosphate pyrophosphohydrolase from human erythrocytes was purified and characterized. The enzyme is highly specific for ITP and shows optimal activity in glycine buffer pH 9.6 and 50 mM MgCl2. The Km of the enzyme is 1.3 X 10(-4), the Vmax = 1.2 X 10(-9) and the Keq = 3.8 X 10(4). Human erythrocyte ITP pyrophosphohydrolase does not require SH compounds for activation. The enzyme is inhibited by Cd++, Co++, and Ca++ ions and by p-hydroxymercuribenzoate.
摘要
相似文献
1
Purification and properties of human erythrocyte inosine triphosphate pyrophosphohydrolase.
J Cell Physiol. 1979 Jan;98(1):41-7. doi: 10.1002/jcp.1040980106.
2
Activity of inosine triphosphate pyrophosphohydrolase in fresh and stored human erythrocytes.
Haematologia (Budap). 1981;14(3):277-83.
3
4
[Accumulation of inosine triphosphate in human erythrocytes as a function of ITP-pyrophosphohydrolase activity].
Acta Haematol Pol. 1983 Jul-Dec;14(3-4):165-71.
5
Relationships between nucleoside triphosphate pyrophosphohydrolase activity and inosine triphosphate accumulation in human erythrocytes.
Can J Biochem. 1976 Oct;54(10):843-7. doi: 10.1139/o76-121.
6
The effect of ITPA polymorphisms on the enzyme kinetic properties of human erythrocyte inosine triphosphatase toward its substrates ITP and 6-Thio-ITP.ITPA基因多态性对人红细胞肌苷三磷酸酶针对其底物ITP和6-硫代-ITP的酶动力学特性的影响。
Nucleosides Nucleotides Nucleic Acids. 2011 Nov;30(11):839-49. doi: 10.1080/15257770.2011.606789.
7
Purine metabolism in normal and high-ITP human erythrocytes. Attempts to evaluate the ability to store the cells.
Biomed Biochim Acta. 1987;46(2-3):S263-7.
8
Inosine di- and triphosphate synthesis in erythrocytes and cell extracts.红细胞和细胞提取物中肌苷二磷酸和三磷酸的合成。
J Cell Physiol. 1979 Jun;99(3):287-301. doi: 10.1002/jcp.1040990303.
9
Molecular basis of the antimutagenic activity of the house-cleaning inosine triphosphate pyrophosphatase RdgB from Escherichia coli.来自大肠杆菌的家务型肌苷三磷酸焦磷酸酶RdgB抗诱变活性的分子基础。
J Mol Biol. 2007 Dec 7;374(4):1091-103. doi: 10.1016/j.jmb.2007.10.012. Epub 2007 Oct 11.
10
Nucleoside triphosphate pyrophosphohydrolase (NTPH).
Methods Enzymol. 1978;51:275-85. doi: 10.1016/s0076-6879(78)51037-9.
引用本文的文献
1
Inosine Triphosphate Pyrophosphatase (ITPase): Functions, Mutations, Polymorphisms and Its Impact on Cancer Therapies.肌苷三磷酸焦磷酸酶（ITPase）：功能、突变、多态性及其对癌症治疗的影响。
Cells. 2022 Jan 24;11(3):384. doi: 10.3390/cells11030384.
2
Measuring deaminated nucleotide surveillance enzyme ITPA activity with an ATP-releasing nucleotide chimera.使用一种释放ATP的核苷酸嵌合体测量脱氨基核苷酸监测酶ITPA的活性。
Nucleic Acids Res. 2017 Nov 16;45(20):11515-11524. doi: 10.1093/nar/gkx774.
3
A disease spectrum for ITPA variation: advances in biochemical and clinical research.ITPA变异的疾病谱：生化与临床研究进展
J Biomed Sci. 2016 Oct 22;23(1):73. doi: 10.1186/s12929-016-0291-y.
4
ITPA (inosine triphosphate pyrophosphatase): from surveillance of nucleotide pools to human disease and pharmacogenetics.ITPA（肌苷三磷酸焦磷酸酶）：从核苷酸池的监测到人类疾病和药物遗传学。
Mutat Res. 2013 Oct-Dec;753(2):131-146. doi: 10.1016/j.mrrev.2013.08.001. Epub 2013 Aug 19.
Zotero 插件