Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA.
Methods Mol Biol. 2021;2230:457-465. doi: 10.1007/978-1-0716-1028-2_29.
The osteoclast is the unique type of cell that resorbs bone in vivo and it is required for normal skeletal development and postnatal homeostasis. Osteoclast deficiency impairs skeletal development during embryogenesis and results in osteopetrosis and impaired tooth eruption. In contrast, excessive osteoclast formation in adults results in bone loss in a number of conditions, including osteoporosis, rheumatoid arthritis, and metastatic bone disease. Osteoclasts are derived from monocytes/macrophages; they can be generated in vitro by treatment of these precursor cells with macrophage colony stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL). This chapter describes procedures for generating osteoclasts from mouse bone marrow cells in vitro using M-CSF and RANKL and assessing their ability to form resorption lacunae on thin bone slices.
破骨细胞是唯一能在体内吸收骨的细胞类型，对于正常的骨骼发育和出生后的稳态是必需的。破骨细胞缺乏会损害胚胎发育过程中的骨骼发育，并导致骨质硬化症和牙齿萌出受损。相反，成年人中过多的破骨细胞形成会导致多种情况下的骨丢失，包括骨质疏松症、类风湿关节炎和转移性骨病。破骨细胞来源于单核细胞/巨噬细胞；这些前体细胞经巨噬细胞集落刺激因子（M-CSF）和核因子-κB 受体激活剂配体（RANKL）处理后，可以在体外生成破骨细胞。本章描述了使用 M-CSF 和 RANKL 从鼠骨髓细胞体外生成破骨细胞的程序，并评估了它们在薄骨切片上形成吸收陷窝的能力。
