Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Agricultural Technology, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand.
In Vitro Cell Dev Biol Anim. 2020 Dec;56(10):842-846. doi: 10.1007/s11626-020-00529-3. Epub 2020 Nov 16.
In general, goat mammary epithelial cells (MECs) are cultured in vitro under 37 °C. We demonstrated previously that goat MECs differentiate under 37 °C although their body temperature is approximately 39 °C. This study aimed to investigate the influence of 39 °C on lactogenic differentiation of goat milk-derived MECs. The results revealed that HSP70 gene was significantly elevated at 1 h after an exposure to 39 °C but declined at 48 h thereafter. Oxidative stress status was not significantly affected by 39 °C. Expressions of CSN2, β-GALT1, α-LA, and Akt genes tended to increase after the differentiation under 39 °C. Secretion of lactose under 39 °C was not significantly lower than 37 °C. In conclusion, incubation temperature at 39 °C does not dramatically affect lactogenic function of goat milk-derived MECs.
通常情况下，山羊乳腺上皮细胞（MEC）在 37°C 下进行体外培养。我们之前的研究表明，尽管山羊的体温约为 39°C，但它们的乳腺上皮细胞在 37°C 下也能分化。本研究旨在探讨 39°C 对羊奶来源的 MEC 泌乳分化的影响。结果表明，HSP70 基因在暴露于 39°C 1 小时后显著升高，但此后 48 小时后下降。39°C 对氧化应激状态没有显著影响。CSN2、β-GALT1、α-LA 和 Akt 基因的表达在 39°C 下分化后趋于增加。39°C 下乳糖的分泌并不明显低于 37°C。综上所述，39°C 的孵育温度不会显著影响羊奶来源的 MEC 的泌乳功能。
