Jetten A M
Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, N.C.
Dermatologica. 1987;175 Suppl 1:37-44. doi: 10.1159/000248852.
The objective of our research is to elucidate the molecular mechanisms that regulate the proliferation and differentiation of tracheobronchial epithelial cells. A clear insight into the basic mechanisms of these processes is required in order to understand the mechanisms involved in tumor development and other pathological conditions. To study the regulation of differentiation of the tracheobronchial epithelium, an in vitro cell culture model was developed using rabbit tracheal epithelial cells. Our studies were directed towards answering the following questions: (1) What is the differentiation capacity of these cells? (2) Does differentiation occur as a multistep process? (3) What factors control differentiation and by what mechanism? (4) What are the biochemical markers for the various differentiated phenotypes and at what level are they controlled? (5) Is there a link between control of cell growth and control of cell differentiation?
我们研究的目的是阐明调节气管支气管上皮细胞增殖和分化的分子机制。为了理解肿瘤发生及其他病理状况所涉及的机制，需要深入了解这些过程的基本机制。为研究气管支气管上皮的分化调控，我们利用兔气管上皮细胞建立了一个体外细胞培养模型。我们的研究旨在回答以下问题：（1）这些细胞的分化能力如何？（2）分化是否作为一个多步骤过程发生？（3）哪些因素控制分化以及通过何种机制？（4）各种分化表型的生化标志物是什么以及它们在何种水平受到控制？（5）细胞生长控制与细胞分化控制之间是否存在联系？
