Tidewater Agricultural Research and Extension Center, Virginia Tech University, Suffolk, VA 23437.
Plant Dis. 2021 Jan;105(1):53-59. doi: 10.1094/PDIS-03-20-0569-RE. Epub 2020 Nov 16.
The tarnished plant bug, (Palisot de Beauvois), is an important insect pest in cotton that feeds on reproductive fruit, contributing to yield loss. Economically damaging infestations of have doubled in Virginia since 2013. Escalation of abundance may increase Fusarium hardlock disease observed in this region, compounding economic losses. Research has linked Fusarium hardlock with fungal species and . Field and greenhouse experiments were performed to investigate (i) Fusarium hardlock occurrence in field plots managed and unmanaged for , (ii) severity of infection of cotton bolls with and without the presence of feeding in a greenhouse setting, and (iii) species composition and prevalence within field-collected and cotton lint with and without insect feeding injury and hardlock symptoms present. Nearly twice the amount of hardlock (i.e., proportion of hardlocked locules) occurred in field-collected bolls with feeding symptoms (0.40 ± 0.02) compared with bolls without (0.21 ± 0.01). Based on real-time quantitative PCR, cotton bolls exposed to inoculum and caged with adults had greater levels of DNA compared with untreated bolls. , , and were isolated from field-collected and hardlocked cotton lint at harvest. These findings suggest that the presence of is associated with an increased risk of Fusarium hardlock in Southeastern cotton, and both should be carefully managed using timely insecticide applications and cultural control practices to minimize yield loss.
玷污叶蝉（Palisot de Beauvois）是棉花上的一种重要害虫，以生殖果为食，导致产量损失。自 2013 年以来，弗吉尼亚州的经济破坏性玷污叶蝉虫害增加了一倍。这种地区观察到的 丰度增加可能会加剧镰刀菌硬锁病，从而加剧经济损失。研究已经将镰刀菌硬锁与真菌物种 和 联系起来。进行了田间和温室实验，以调查：（i）管理和未管理玷污叶蝉的田间小区中镰刀菌硬锁的发生情况；（ii）温室环境中有无玷污叶蝉取食时棉花铃的感染严重程度；（iii）田间采集的玷污叶蝉和棉花纤维中 和 物种组成和流行情况，以及有无昆虫取食损伤和硬锁症状。与无玷污叶蝉取食症状的棉铃（0.21 ± 0.01）相比，有玷污叶蝉取食症状的田间采集棉铃中硬锁的比例（即硬锁小室的比例）几乎高出一倍（0.40 ± 0.02）。基于实时定量 PCR，暴露于 接种体并用 成虫笼养的棉花铃比未处理的棉花铃具有更高水平的 DNA。在收获时，从田间采集的玷污叶蝉和硬锁棉花纤维中分离出了 、 和 。这些发现表明，玷污叶蝉的存在与东南棉花中镰刀菌硬锁的风险增加有关，应通过及时使用杀虫剂和文化控制措施来仔细管理 ，以最大程度地减少产量损失。
