Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, 266071, China.
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
Aquat Toxicol. 2020 Dec;229:105678. doi: 10.1016/j.aquatox.2020.105678. Epub 2020 Nov 7.
The rotifer, Brachionus plicatilis, is a widely used model species in marine ecotoxicology for evaluating pollutions, toxins, and harmful algae. In this paper, the marine ecotoxicology of Brachionus plicatilis was reviewed, including toxicity measurements of harmful algae species and environmental stresses. In addition, marine pollution involving pesticides, heavy metals, drugs, petroleum, and petrochemicals were addressed. Methods for measuring toxicity were also discussed. The standard acute lethal assay and the chronic population dynamics test were indicated as common methods of toxicity evaluating using B. plicatilis. Research on other biomarkers, such as behaviour, enzyme activity, or gene expression, are also reported here, with potential applications for fast detection or the scientific exploration of underlying molecular mechanisms. It is suggested that the methods selected should reflect the experimental purpose. Additionally, series assays should be conducted for comprehensive evaluation of ecotoxicity as well as to elucidate the correct mechanisms. Genetic methods, such as transcriptomics, were suggested as useful tools for exploring the toxicity mechanism using the rotifer B. plicatilis.
褶皱臂尾轮虫是海洋生态毒理学中广泛应用的模式生物，用于评估污染、毒素和有害藻类。本文综述了褶皱臂尾轮虫的海洋生态毒理学，包括有害藻类物种和环境胁迫的毒性测量。此外，还涉及了涉及农药、重金属、药物、石油和石油化工的海洋污染。还讨论了测量毒性的方法。标准急性致死测定和慢性种群动态测试被认为是使用褶皱臂尾轮虫评估毒性的常见方法。本文还报道了其他生物标志物，如行为、酶活性或基因表达的研究，这些方法具有快速检测或科学探索潜在分子机制的应用潜力。建议选择的方法应反映实验目的。此外，还应进行一系列测定，以全面评估生态毒性，并阐明正确的机制。遗传方法，如转录组学，被建议作为使用轮虫褶皱臂尾轮虫探索毒性机制的有用工具。
