Department of Biology, Faculty of Natural and Computational Science, Kotebe Metropolitan University, Addis Ababa, Ethiopia.
Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia.
BMC Biotechnol. 2020 Nov 16;20(1):60. doi: 10.1186/s12896-020-00651-w.
Moringa stenopetala belongs to the flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The expansion of its cultivation and utilization throughout the world especially in Africa is becoming important. For such expansion, the existing propagation method is limiting, so it needs a good propagation system to supply enough planting material with a uniform genotype. Therefore, the main objective of this study was to optimize an in vitro shoot multiplication protocol for M. stenopetala by using shoot tip as explants.
Shoots were sterilized and cultured on Muraghige and Skoog (MS) medium for in vitro shoot initiation. For multiple shoot induction, the explants were cultured on MS medium supplemented with different concentrations of kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/L) with Indole-3- butyric acid (IBA) or α -naphthalene acetic acid (NAA) (0.01, 0.1, 0.5 mg/L) and maintained at 25 ± 2 °C for four weeks. Rooting was achieved by culturing well developed shoots in half-strength MS medium containing IBA (0.1, 0.5, 1.0, 1.5, 2.0 mg/L), NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/L), and 0.5 mg/L IBA with NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/L). Statistical analysis revealed that there was a significant difference among all treatments applied in both shoot multiplication and rooting experiments. The maximum number of shoots per explant (3.43 ± 1.41) and 7.97 ± 4.18 leaves per explant were obtained on MS medium containing 0.5 mg/L kinetin with 0.01 mg/LNAA. The highest mean number of roots per shoot (1.63 ± 1.03) and mean root length (0.87 ± 1.22 cm) were obtained on MS medium containing 1.0 mg/LNAA and 0.1 mg/LIBA alone respectively. After acclimatization, 76% of plants were survived in the greenhouse.
In general, using NAA with kinetin for shoot multiplication was effective than kinetin with IBA. On the other hand, the application of 1.0 mg/L NAA alone and 1.0 mg/L NAA with 0.5 mg/L IBA were more effective for root induction.
辣木属（Moringa stenopetala）属于辣木科（Moringaceae）辣木属（Moringa）。它通常被称为东非辣木树，因为它仅原产于埃塞俄比亚南部和肯尼亚北部。它的种植和利用在世界各地，特别是在非洲的扩张变得越来越重要。对于这种扩张，现有的繁殖方法是有限的，因此需要一个良好的繁殖系统来提供足够的具有均匀基因型的种植材料。因此，本研究的主要目的是使用茎尖作为外植体来优化辣木属的离体芽增殖方案。
将芽在 Muraghige 和 Skoog（MS）培养基上进行消毒和培养，以进行离体芽起始。为了诱导多个芽，将外植体在 MS 培养基上培养，该培养基补充有不同浓度的激动素（0.5、1.0、1.5、2.0、2.5mg/L），吲哚丁酸（IBA）或α-萘乙酸（NAA）（0.01、0.1、0.5mg/L），并在 25±2°C 下保持四周。通过将发育良好的芽在含有 IBA（0.1、0.5、1.0、1.5、2.0mg/L）、NAA（0.1、0.5、1.0、1.5、2.0mg/L）和 0.5mg/L IBA 的半强度 MS 培养基中培养来实现生根，NAA（0.1、0.5、1.0、1.5、2.0mg/L）。统计分析表明，在芽增殖和生根实验中，所有处理之间均存在显著差异。在含有 0.5mg/L 激动素和 0.01mg/L NAA 的 MS 培养基上，每个外植体获得的芽数最多（3.43±1.41）和每个外植体的叶片数最多（7.97±4.18）。每个芽获得的平均生根数（1.63±1.03）和平均根长（0.87±1.22cm）最高，是在含有 1.0mg/L NAA 和单独 0.1mg/L IBA 的 MS 培养基上获得的。在适应环境后，温室中有 76%的植物存活。
总的来说，使用 NAA 加激动素进行芽增殖比使用激动素加 IBA 更有效。另一方面，单独使用 1.0mg/L NAA 和单独使用 1.0mg/L NAA 加 0.5mg/L IBA 更有利于诱导生根。
