Department of Biology, College of Natural and Computational Sciences, Dilla University, Dilla 419, Ethiopia.
ScientificWorldJournal. 2021 Feb 8;2021:5928769. doi: 10.1155/2021/5928769. eCollection 2021.
Potato ( L.) is one of the important crops in Ethiopia which has a crucial role in nutritional security, poverty alleviation, and income generation. The aim of the present investigation is to develop an efficient propagation protocol for Belete and Gudiene potato varieties by using lateral bud as explants.
Shoot initiation was achieved by inoculating buds on full-strength MS Murashige and Skoog medium (MS) fortified with variable concentrations of BAP and NAA. Basal MS was used as control throughout the experiment.
Results of our study showed that best shoot initiation was obtained on MS medium supplemented with 1.5 mg/l BAP + 3.0 mg/l NAA for Gudiene variety, whereas 1.0 mg/l BAP and 2.0 mg/l NAA produced more shoots in Belete variety. The initiated shoots increased two- to three-fold upon subculture on the MS medium fortified with varying concentrations of BAP and Kinetin. The highest numbers of multiple shoots were obtained in the MS medium containing 2.5 mg/l Kinetin. The combined effect of BAP and Kinetin did not produce any additional positive effect for shoot multiplication. Rooting percentage and number of roots/shoot were found best on the MS medium fortified with 1.0 mg/l IBA + 0.5 IAA.
The variety Gudiene was found best for shoot initiation and root formation, while Belete variety proved its superiority for multiple shoot formation. A total number of 82.66% of plantlets were acclimatized under field conditions. This work indicates the practical applicability of plant tissue culture using lateral bud as explants is effective for micropropagation of potato .
马铃薯（L.）是埃塞俄比亚的重要作物之一，在营养安全、减贫和增收方面发挥着关键作用。本研究旨在利用侧芽作为外植体，为 Belete 和 Gudiene 马铃薯品种开发一种高效的繁殖方案。
通过在添加了不同浓度 BAP 和 NAA 的完全 MS Murashige 和 Skoog 培养基（MS）上接种芽，实现了芽的起始。整个实验过程中，基础 MS 培养基作为对照。
研究结果表明，对于 Gudiene 品种，最佳芽起始是在添加 1.5 mg/l BAP + 3.0 mg/l NAA 的 MS 培养基上获得的，而在 Belete 品种中，1.0 mg/l BAP 和 2.0 mg/l NAA 产生了更多的芽。在添加不同浓度 BAP 和激动素的 MS 培养基上进行继代培养后，起始芽增加了两到三倍。在含有 2.5 mg/l 激动素的 MS 培养基中获得了最多的多个芽。BAP 和激动素的组合效应对芽的增殖没有产生任何额外的积极影响。在添加 1.0 mg/l IBA + 0.5 IAA 的 MS 培养基上，生根率和根/芽数最佳。
Gudiene 品种最适合芽的起始和根的形成，而 Belete 品种则证明其在多个芽的形成方面具有优势。在田间条件下，有 82.66%的植物可以适应。这项工作表明，使用侧芽作为外植体的植物组织培养在马铃薯的微繁殖中具有实际应用价值。
