College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Key Laboratory of Food Microbiology and Enzyme Engineering Technology of Fujian Province, Xiamen 361021, China; Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Food Chem. 2021 Apr 16;342:128565. doi: 10.1016/j.foodchem.2020.128565. Epub 2020 Nov 7.
The aroma changes in instant white tea resulting from β-glucosidase treatment was investigated by quantitative descriptive analysis (QDA), gas chromatography-mass spectrometry (GC-MS), odour activity value analysis (OAV), aroma reconstruction and omission tests. The grassy, floral and sweet notes increased significantly (P < 0.05), and the roasted note decreased significantly (P < 0.05) upon β-glucosidase treatment. Quantitative analysis showed that the concentrations of benzaldehyde, benzeneacetaldehyde, (Z)-3-hexen-1-ol, linalool, phenylethyl alcohol, cis-linalool oxide, trans-linalool oxide, hexanol, hotrienol and (E)-2-hexen-1-ol increased significantly (P < 0.05) after treatment; however, (Z)-3-hexen-1-ol isomerized to (E)-2-hexen-1-ol. OAV analysis, aroma reconstruction and the omission test showed that the grassy, floral and sweet notes increased as the (Z)-3-hexen-1-ol, cis/trans-linalool oxide and benzeneacetaldehyde increased, whereas the roasted note declined under the same conditions. The enzymatic hydrolysis of glycosidic precursors and the auto-isomerization of volatile compounds provide new information for understanding how β-glucosidase treatment improves the aroma of tea products.
研究了β-葡萄糖苷酶处理对白茶香气的影响，采用定量描述分析（QDA）、气相色谱-质谱联用（GC-MS）、气味活度值分析（OAV）、香气重构和缺失试验进行分析。β-葡萄糖苷酶处理后，青草、花香和甜味显著增加（P < 0.05），烘烤味显著降低（P < 0.05）。定量分析表明，苯甲醛、苯乙醛、（Z）-3-己烯-1-醇、芳樟醇、苯乙醇、顺式-氧化芳樟醇、反式-氧化芳樟醇、己醇、热rienol 和（E）-2-己烯-1-醇的浓度显著增加（P < 0.05）；然而，（Z）-3-己烯-1-醇异构化为（E）-2-己烯-1-醇。OAV 分析、香气重构和缺失试验表明，随着（Z）-3-己烯-1-醇、顺/反式氧化芳樟醇和苯乙醛的增加，青草、花香和甜味增加，而在相同条件下烘烤味下降。糖苷前体的酶水解和挥发性化合物的自动异构化提供了新的信息，有助于理解β-葡萄糖苷酶处理如何改善茶产品的香气。
