IRF6、MSX1、TGFA、牙齿异常和骨骼错颌。

IRF6, MSX1, TGFA, dental anomalies, and skeletal malocclusion.

机构信息

Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Brazil.

Department of Pediatric Dentistry, University of Pittsburgh, PA, USA.

出版信息

Eur J Orthod. 2021 Aug 3;43(4):478-485. doi: 10.1093/ejo/cjaa064.

DOI:10.1093/ejo/cjaa064

PMID:33200192

Abstract

OBJECTIVE

Verify the presence of association between four variables-transforming growth factor α (TGFA; C/T rs1523305), interferon regulatory factor 6 (IRF6; A/C rs2013162), muscle segment homeobox 1 (MSX1; A/G rs12532), and dental anomalies-with skeletal malocclusion by comparing these four variables with Angle Classes I, II, and III, and normal, hyperdivergent, and hypodivergent growth patterns.

METHODS

A total of 505 orthodontic records of patients older than 8 years were evaluated. The sample consisted of 285 (56.4 per cent) females, 220 (43.6 per cent) males, 304 (60.2 per cent) Whites (the rest were mixed Blacks with Whites), with a mean age of 20.28 (±10.35) years (ranging from 8 to 25 years). Eight cephalometric points, which served as the anatomical framework for obtaining angles and cephalometric measurements, were used for skeletal characterization using the Dolphin Software. Samples of saliva were collected and the DNA was extracted, diluted and quantified. Markers in TGFA, IRF6, and MSX1 were used and genotypes were obtained using TaqMan chemistry. Odds ratio (OR) and 95 per cent confidence interval (CI) calculations, chi-square, Fisher's Exact, Mann-Whitney, and correlation coefficient tests (significance level: 95 per cent) were performed. Bonferroni correction was applied and an alpha of 0.0006 was considered statistically significant.

RESULTS

There was no statistically significant associations between markers in TGFA or IRF6 with skeletal malocclusions. Tooth agenesis was associated with facial convexity (P < 0.001). MSX1 was associated with Class II skeletal malocclusion (P = 0.0001, OR = 0.6, CI = 0.46-0.78).

CONCLUSION

Individuals with tooth agenesis were more likely to have a convex face. MSX1 was associated with Class II skeletal malocclusion.

摘要

目的

通过比较转化生长因子α（TGFA；C/T rs1523305）、干扰素调节因子 6（IRF6；A/C rs2013162）、肌肉节同源框 1（MSX1；A/G rs12532）和牙齿异常与 Angle 类Ⅰ、Ⅱ和Ⅲ，以及正常、高角型和低角型生长模式，验证这四个变量与骨骼错畸形之间的相关性。

方法

共评估了 505 例年龄大于 8 岁的正畸患者的记录。样本包括 285 名（56.4%）女性和 220 名（43.6%）男性，304 名（60.2%）白人（其余为白人与黑人混血儿），平均年龄为 20.28（±10.35）岁（8 至 25 岁）。使用 Dolphin 软件，通过 8 个头影测量点作为获得角度和头影测量值的解剖框架，对骨骼特征进行了描述。采集唾液样本并提取 DNA，进行稀释和定量。使用 TaqMan 化学法检测 TGFA、IRF6 和 MSX1 中的标记物并获取基因型。进行了比值比（OR）和 95%置信区间（CI）计算、卡方检验、Fisher 精确检验、Mann-Whitney 检验和相关系数检验（显著性水平：95%）。应用 Bonferroni 校正，将 0.0006 视为具有统计学意义。