Chakrabarty S, Brattain M G, Ochs R L, Varani J
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.
J Cell Physiol. 1987 Dec;133(3):415-25. doi: 10.1002/jcp.1041330302.
The functional relationship between membrane/cell surface expression of fibronectin and laminin and transformation/differentiation was examined in an AKR mouse fibroblastic cell model. This model consisted of the untransformed AKR-2B cells, their chemically transformed counterpart (AKR-MCA cells) and the chemically differentiated form of the AKR-MCA cells. The transformed AKR-MCA cells were found to express more surface laminin and less fibronectin than the untransformed AKR-2B cells. The transformed AKR-MCA cells were slower to attach and spread on both plastic and type IV collagen-coated dishes in comparison to the AKR-2B cells. However, a higher percentage of the AKR-MCA cells ultimately attached and spread on the type IV collagen-coated dishes. The induction of differentiation in the AKR-MCA cells by N,N-dimethylformamide (DMF) restored fibronectin to the surface of the AKR-MCA cells but reduced laminin expression only slightly. The DMF-treated AKR-MCA cells resembled the AKR-2B cells in that they rapidly attached and spread on plastic dishes and dishes coated with type IV collagen. They also resembled the AKR-MCA cells in that a high proportion ultimately attached and spread on the collagen-coated dishes.
在AKR小鼠成纤维细胞模型中，研究了纤连蛋白和层粘连蛋白的膜/细胞表面表达与转化/分化之间的功能关系。该模型由未转化的AKR-2B细胞、其化学转化的对应物（AKR-MCA细胞）以及AKR-MCA细胞的化学分化形式组成。发现转化后的AKR-MCA细胞比未转化的AKR-2B细胞表达更多的表面层粘连蛋白和更少的纤连蛋白。与AKR-2B细胞相比，转化后的AKR-MCA细胞在塑料和IV型胶原包被的培养皿上附着和铺展的速度较慢。然而，更高比例的AKR-MCA细胞最终在IV型胶原包被的培养皿上附着和铺展。用N，N-二甲基甲酰胺（DMF）诱导AKR-MCA细胞分化，可使纤连蛋白恢复到AKR-MCA细胞表面，但仅略微降低层粘连蛋白的表达。经DMF处理的AKR-MCA细胞与AKR-2B细胞相似，它们能在塑料培养皿和IV型胶原包被的培养皿上迅速附着和铺展。它们也与AKR-MCA细胞相似，即有很大比例的细胞最终在胶原包被的培养皿上附着和铺展。
