Department of Plastic Surgery, Peking University Third Hospital, Beijing 100191, P.R. China.
Mol Med Rep. 2021 Jan;23(1). doi: 10.3892/mmr.2020.11685. Epub 2020 Nov 17.
Dedifferentiated adipose cells (DAs) and adipose‑derived stem cells (ADSCs) are two of the primary types of stem cells derived from adipose tissue, which have been reported to possess similar characteristics, but also exhibit unique phenotypic and functional advantages. However, several reports have described inconsistent results regarding their differences in multilineage differentiation function. Moreover, to the best of our knowledge, there are no studies assessing their myogenic ability, or the differences in the transcriptome between the two cell types derived from lipoaspirates via tumescent liposuction from the same donors. The aim of the present study was to compare the properties and expression profiles of these cell types. Subcutaneous adipose tissue of three female patients (aged 23‑30 years) with a physiological BMI (19.1‑23.9 kg/m) were obtained during tumescent liposuction of the abdomen or the thigh. The stromal vascular fraction and mature adipocytes were obtained via collagenase digestion, and ADSCs and DAs were cultured successively. To determine the differences between DAs and ADSCs after 6‑7 passages, cell proliferation assays, phenotypic assessment, differentiation assays and high‑throughput RNA sequencing (seq) were used. Similar cell morphologies, proliferation dynamics, surface markers and transcriptome expression profiles were observed between the DAs and ADSCs. Whilst there were notable individual differences in the osteogenic, lipogenic, chondrogenic and myogenic abilities of the DAs and ADSCs, it was difficult to determine their differentiation potential based only on the cell source. Interestingly, the myogenic ability was relatively stronger in cells with relatively weaker lipogenic ability. Only 186 differentially expressed genes between the two groups were identified using RNAseq. Several of these genes were involved in biological functions such as transcription regulation, protein translation regulation, cytokine interactions and energy metabolism regulation. The results of the present study suggested a similar functional potential of DAs and ADSCs from young donors undergoing tumescent liposuction operation in regeneration areas and the balance of the differentiative ability of the same cell populations. These data may provide a foundation for further clinical administration of stem cells derived from adipose tissues in therapy.
去分化脂肪细胞（DAs）和脂肪来源的干细胞（ADSCs）是两种主要的来源于脂肪组织的干细胞，据报道它们具有相似的特征，但也表现出独特的表型和功能优势。然而，有几项报道描述了它们在多谱系分化功能上的差异不一致的结果。此外，据我们所知，尚无研究评估这两种细胞类型的成肌能力，或者同一供体来源的肿胀吸脂术获取的脂肪抽吸物中，这两种细胞类型之间的转录组差异。本研究旨在比较这两种细胞类型的特性和表达谱。在腹部或大腿的肿胀吸脂术过程中，从 3 名生理 BMI（19.1-23.9kg/m）的女性患者（年龄 23-30 岁）的皮下脂肪组织中获得基质血管部分和成熟脂肪细胞，通过胶原酶消化获得 ADSCs 和 DAs，并连续培养。为了确定 6-7 代后 DAs 和 ADSCs 之间的差异，进行了细胞增殖试验、表型评估、分化试验和高通量 RNA 测序（seq）。在 DAs 和 ADSCs 之间观察到相似的细胞形态、增殖动力学、表面标志物和转录组表达谱。虽然 DAs 和 ADSCs 的成骨、成脂、成软骨和成肌能力存在明显的个体差异，但仅根据细胞来源很难确定它们的分化潜力。有趣的是，具有相对较弱成脂能力的细胞具有相对较强的成肌能力。仅使用 RNAseq 鉴定了两组之间的 186 个差异表达基因。其中一些基因参与了转录调控、蛋白质翻译调控、细胞因子相互作用和能量代谢调控等生物学功能。本研究的结果表明，在接受肿胀吸脂术的年轻供体的再生区域中，DAs 和 ADSCs 具有相似的功能潜力，以及同一细胞群体的分化能力的平衡。这些数据可能为进一步临床管理脂肪组织来源的干细胞在治疗中的应用提供基础。
