一种用于同时检测抗坏血酸、多巴胺、尿酸和亚硝酸盐的多功能电化学传感器。

A Multifunctional Electrochemical Sensor for the Simultaneous Detection of Ascorbic Acid, Dopamine, Uric Acid, and Nitrite.

作者信息

Wang Huimin, Zhang Xueli, Wang Shuangjue, Ma Hanyue, Shen Yaling, Wang Xia

机构信息

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, P.R. China.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, P.R. China.

出版信息

J AOAC Int. 2021 Jun 12;104(3):860-866. doi: 10.1093/jaoacint/qsaa157.

DOI:10.1093/jaoacint/qsaa157

PMID:33201187

Abstract

BACKGROUND

Ascorbic acid (AA), dopamine (DA), uric acid (UA), and nitrite (NO2-) are essential biomarkers for human metabolism and can be used to indicate some chronic diseases and metabolic disorders, including scurvy, Parkinson's disease, hyperuricemia, and kidney disease.

OBJECTIVE

A multifunctional electrochemical sensor that can integrate the detection of these species was constructed using nanoporous gold (NPG) as a recognition element to modify glassy carbon electrode (GCE).

METHODS

The electrochemical performance of the multifunctional electrochemical sensor was investigated toward AA, DA, UA, and NO2- in citrate buffer solution (CBS, 100 mM, pH 4.0) and human serum using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods.

RESULTS

In the quaternary mixture detection, the resulting NPG/GCE electrode displayed four independent oxidation peaks with wide peak separations. Further, the NPG/GCE electrode showed good linear responses with the sensitivities of 32, 1103, 71, and 147 μA/mM/cm2 and the detection limits of 1.58, 0.17, 0.37, and 0.36 μM for AA, DA UA, and NO2-, respectively. Additionally, the NPG/GCE electrode exhibited great anti-interference and was successfully applied in human serum samples.

CONCLUSIONS

These results indicate that the NPG/GCE electrode can simultaneously and selectively detect AA, DA, UA, and NO2-, which has the potential for application and diagnosis in the screening and diagnosis of chronic diseases and metabolic disorders.

HIGHLIGHTS

A multianalyte electrochemical sensor was fabricated for human metabolites detection. The sensor displayed good performance in the simultaneous detection of AA, DA, UA, and NO2- and applied to human serum samples.

摘要

背景

抗坏血酸（AA）、多巴胺（DA）、尿酸（UA）和亚硝酸盐（NO₂⁻）是人体新陈代谢的重要生物标志物，可用于指示某些慢性疾病和代谢紊乱，包括坏血病、帕金森病、高尿酸血症和肾脏疾病。

目的

构建一种多功能电化学传感器，该传感器能够整合对这些物质的检测，采用纳米多孔金（NPG）作为识别元件修饰玻碳电极（GCE）。

方法

使用循环伏安法（CV）和差分脉冲伏安法（DPV）研究多功能电化学传感器在柠檬酸盐缓冲溶液（CBS，100 mM，pH 4.0）和人血清中对AA、DA、UA和NO₂⁻的电化学性能。

结果

在四元混合物检测中，所得的NPG/GCE电极显示出四个独立的氧化峰，峰间距较宽。此外，NPG/GCE电极表现出良好的线性响应，对AA、DA、UA和NO₂⁻的灵敏度分别为32、1103、71和147 μA/mM/cm²，检测限分别为1.58、0.17、0.37和0.36 μM。此外，NPG/GCE电极具有很强的抗干扰能力，并成功应用于人血清样本。