原位杂交-邻近连接检测（ISH-PLA）用于研究 HIV-1 RNA 与重塑蛋白的相互作用。
In Situ Hybridization-Proximity Ligation Assay (ISH-PLA) to Study the Interaction of HIV-1 RNA and Remodeling Proteins.
机构信息
Instituto de Ciencias Biomedicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago, Chile.
Laboratory of Molecular and Cellular Virology, Virology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
出版信息
Methods Mol Biol. 2021;2209:307-319. doi: 10.1007/978-1-0716-0935-4_19.
The mechanisms involved in the posttranscriptional control of the replicative cycle of the human immunodeficiency virus (HIV), specifically the molecular events which allow the interaction between the viral genomic RNA (gRNA) and the cellular machinery for the transport, translation, or intracellular packaging, have not been yet elucidated. In this chapter, we describe the in situ hybridization-proximity ligation assay (ISH-PLA) to characterize interactions between the genomic RNA (gRNA) of HIV-1 and viral proteins or host proteins involved in nuclear export and translation initiation. We also present data that validate the ISH-PLA as a simple and useful tool to study HIV-1 gRNA-protein interactions within cells.
HIV 复制周期的转录后控制机制，特别是允许病毒基因组 RNA（gRNA）与细胞运输、翻译或细胞内包装的机制相互作用的分子事件，尚未阐明。在本章中，我们描述了原位杂交-邻近连接分析（ISH-PLA），以表征 HIV-1 的基因组 RNA（gRNA）与参与核输出和翻译起始的病毒蛋白或宿主蛋白之间的相互作用。我们还提供了数据，验证了 ISH-PLA 作为一种简单而有用的工具，可用于研究细胞内 HIV-1 gRNA-蛋白相互作用。
Zotero 插件