State Key Laboratory of Refractories and Metallurgy, Key Laboratory of Coal Conversion & New Carbon Materials of Hubei Province, Wuhan University of Science and Technology, Wuhan 430081, P. R. China.
Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, P. R. China.
Langmuir. 2020 Dec 1;36(47):14268-14275. doi: 10.1021/acs.langmuir.0c02393. Epub 2020 Nov 17.
Inorganic nanosheets are endowed with many two-dimensional (2D) morphological features including ultra-high specific surface area, ultra-thin thickness, easy functionalization, and so on. They push forward an immense influence on effective cancer diagnosis and therapy, overcoming the inherent limitations of traditional treatment methods. However, long-term toxicity and poor biocompatibility are the critical issues for most inorganic nanosheets, which hinder their further oncological applications and clinical translations. Muscovite, also named white mica (WM), an aluminosilicate, is a major component of traditional Chinese medicine, which can be exfoliated into 2D nanosheets and expected to be a potential drug carrier. In this study, WM powder was exfoliated to prepare WM nanosheets (WMNs) through a polyamine intercalation method. In addition, doxorubicin hydrochloride (Dox) was loaded to WMNs physical adsorption and electrostatic interaction to prepare Dox-loaded WMNs (Dox@WMNs). Then, we studied that Dox@WMNs released Dox in phosphate buffer saline. We also studied the cellular uptake and cytotoxicity of Dox@WMNs . The results illustrated that Dox@WMNs cumulatively released Dox much faster and more at acidic pH (6.0 and 4.6) compared with that at physiological pH. In addition, WMNs showed selective cytotoxicity. Within a certain concentration range, WMNs were cytotoxic to Hela cells but non-cytotoxic to RAW 264.7 cells. Compared with cytotoxicity at pH 7.4, the cytotoxicity of Dox@WMNs was significantly enhanced at pH 6.4 and 4.6. WMNs mainly promoted the immunostimulatory polarization of RAW 264.7 cells into M1 macrophages.
无机纳米片具有许多二维（2D）形态特征，包括超高比表面积、超薄厚度、易于功能化等。它们对有效的癌症诊断和治疗产生了巨大的影响，克服了传统治疗方法的固有局限性。然而，大多数无机纳米片的长期毒性和较差的生物相容性是关键问题，这阻碍了它们在肿瘤学中的进一步应用和临床转化。白云母，也称为白云母（WM），是一种铝硅酸盐，是中药的主要成分，可被剥离成 2D 纳米片，有望成为一种潜在的药物载体。在本研究中，通过多胺插层法将白云母粉末剥离制备得到白云母纳米片（WMNs）。此外，通过物理吸附和静电相互作用将盐酸阿霉素（Dox）负载到 WMNs 上，制备得到载药 WMNs（Dox@WMNs）。然后，我们研究了 Dox@WMNs 在磷酸盐缓冲盐水中释放 Dox 的情况。我们还研究了 Dox@WMNs 的细胞摄取和细胞毒性。结果表明，与生理 pH 相比，在酸性 pH（6.0 和 4.6）条件下，Dox@WMNs 更快、更多地累积释放 Dox。此外，WMNs 表现出选择性细胞毒性。在一定浓度范围内，WMNs 对 Hela 细胞具有细胞毒性，但对 RAW 264.7 细胞无细胞毒性。与 pH 7.4 时的细胞毒性相比，Dox@WMNs 在 pH 6.4 和 4.6 时的细胞毒性显著增强。WMNs 主要促进 RAW 264.7 细胞向 M1 巨噬细胞的免疫刺激极化。
