Gao Qiang, Yu Guihong, Yu Minghui, Wang Xinping
Department of Spleen and Stomach Disease, Yantai Hospital of Traditional Chinese Medicine, Yantai 264002, China.
Department of Integrated TCM & Western Medicine, Yantai Qishan Hospital, Yantai 264000, China.
J Oncol. 2020 Oct 28;2020:3531652. doi: 10.1155/2020/3531652. eCollection 2020.
We aimed to explore the effects of Bupleuri Radix (BR) on the recurrence of resected colonic polyp (CP) by measuring angiogenin-2-induced protein kinase B (Ang PKB)/Akt signaling.
The main ingredients of BR were extracted by using ethanol and measured by HPLC. One hundred twenty patients with CP >10 mm who underwent resected surgery were randomly allocated to an aspirin (AG) or a BR medicine (BG) group. The allocation ratio was 1 : 1 and the intervention duration was one year. The recurrence rate of resected CP was investigated and the plasma levels of Ang PKB/Akt and inflammatory cytokines were measured using ELISA kits. After one-year surgery, side effects were recorded. The relationship between the serum levels of the main compounds of BR and plasma levels of Ang PKB/Akt was analyzed.
The main ingredients of CP are paeoniflorin, baicalin, saikosaponin A, and bupleurum saponin B2. Recurrence of resected CP was found in 17 patients from the AG group and eight patients from the BG group after one-year follow-up ( < 0.05). The levels of angiogenin-2 II and PKB/Akt in the AG group were higher than those in the BG group ( < 0.05). Meanwhile, BR treatment reduced the plasma levels of TNF-, IL-1 and IL-6, and increased the level of IL-10( < 0.05). Inflammatory cytokines are important factors that affect the recurrence of resected CP. Serum paeoniflorin, baicalin, saikosaponin A, and bupleurum saponin B2 in BR had a strong negative relationship with the plasma levels of Ang PKB/Akt.
BR significantly reduces the recurrence risk of resected CP by affecting Ang PKB/Akt signaling.
通过检测血管生成素 -2 诱导的蛋白激酶 B（Ang PKB）/Akt 信号通路，探讨柴胡对切除结肠息肉（CP）复发的影响。
采用乙醇提取柴胡的主要成分并用高效液相色谱法进行测定。120 例 CP 直径大于 10 毫米且接受了切除手术的患者被随机分为阿司匹林（AG）组或柴胡药物（BG）组。分配比例为 1∶1，干预持续时间为一年。研究切除 CP 的复发率，并使用酶联免疫吸附测定试剂盒测量 Ang PKB/Akt 和炎性细胞因子的血浆水平。手术一年后，记录副作用。分析柴胡主要化合物的血清水平与 Ang PKB/Akt 血浆水平之间的关系。
CP 的主要成分是芍药苷、黄芩苷、柴胡皂苷 A 和柴胡皂苷 B2。一年随访后，AG 组有 17 例患者出现切除 CP 复发，BG 组有 8 例患者复发（<0.05）。AG 组血管生成素 -2 II 和 PKB/Akt 的水平高于 BG 组（<0.05）。同时，柴胡治疗降低了血浆肿瘤坏死因子 -、白细胞介素 -1 和白细胞介素 -6 的水平，并提高了白细胞介素 -10 的水平（<0.05）。炎性细胞因子是影响切除 CP 复发的重要因素。柴胡中的血清芍药苷、黄芩苷、柴胡皂苷 A 和柴胡皂苷 B2 与 Ang PKB/Akt 的血浆水平呈强负相关。
柴胡通过影响 Ang PKB/Akt 信号通路显著降低切除 CP 的复发风险。
